TY - JOUR
T1 - Blood compatibility and tissue responsiveness on simple and durable methylsiloxane coating
AU - Hoshikawa, Yasuto
AU - Onoki, Takamasa
AU - Akao, Masaru
AU - Akatsu, Takashi
AU - Tanabe, Yasuhiro
AU - Yasuda, Eiichi
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Cooperative Research Project of Nationwide Joint-Use Research Institutes on Development Base of Joining Technology for New Metallic Glasses and the Collaborative Research Project of Materials and Structures Laboratory , Tokyo Institute of Technology . The authors are grateful to Mr. T. Onoda (Tokyo Institute of Technology) for the helpful measurement. Y.H. acknowledges the partial support from a Grant-in-Aid for Young Scientists (B) ( 21760527 ) from MEXT, Japan and Collaborative Research Project of Materials and Structures Laboratory , Tokyo Institute of Technology . The authors appreciated Dr. S. Katsuta and his co-workers from the foundation of Japan Food Research Laboratories for their help with the blood and animal tests.
PY - 2012/8/1
Y1 - 2012/8/1
N2 - This study was conducted to evaluate the blood compatibility and tissue responsiveness of methylsiloxane (MS)-coated inorganic (glass and metal) substrates both in vitro and in vivo. MS was prepared from methyltriethoxysilane (MTES) through hydrolysis of a sol-gel solution at 80°C. The adhesive strength of the MS coating was evaluated by using a tear-off test, revealing that the MS strongly adhered to the surface of the inorganic substrates. Blood compatibility was evaluated by assessing platelet adhesion and blood plasma coagulation time. The platelet aggregation ratio of the MS-coated glass tube was reduced to 10%, which was much smaller than that of the coating-free glass tubes (99%) and conventional blood-compatible polystyrene (PS) tubes (18%). Coagulation time was measured by active partial thromboplastin time (APTT) test, which showed that MS coating is as inert as PS in activating blood coagulation factor XII. Tissue responsiveness to the bulk MS sample, evaluated by animal test, showed a desirable compatibility comparable to that of the control titanium sample. This study indicated that MS coating is readily available to convert inorganic materials to useful biomaterials that have suitable mechanical strength and are compatible with blood and tissue.
AB - This study was conducted to evaluate the blood compatibility and tissue responsiveness of methylsiloxane (MS)-coated inorganic (glass and metal) substrates both in vitro and in vivo. MS was prepared from methyltriethoxysilane (MTES) through hydrolysis of a sol-gel solution at 80°C. The adhesive strength of the MS coating was evaluated by using a tear-off test, revealing that the MS strongly adhered to the surface of the inorganic substrates. Blood compatibility was evaluated by assessing platelet adhesion and blood plasma coagulation time. The platelet aggregation ratio of the MS-coated glass tube was reduced to 10%, which was much smaller than that of the coating-free glass tubes (99%) and conventional blood-compatible polystyrene (PS) tubes (18%). Coagulation time was measured by active partial thromboplastin time (APTT) test, which showed that MS coating is as inert as PS in activating blood coagulation factor XII. Tissue responsiveness to the bulk MS sample, evaluated by animal test, showed a desirable compatibility comparable to that of the control titanium sample. This study indicated that MS coating is readily available to convert inorganic materials to useful biomaterials that have suitable mechanical strength and are compatible with blood and tissue.
KW - Blood compatibility
KW - Coating
KW - Methyltriethoxysilane
KW - Sol-gel
KW - Tissue responsiveness
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U2 - 10.1016/j.msec.2012.04.053
DO - 10.1016/j.msec.2012.04.053
M3 - Article
C2 - 24364969
AN - SCOPUS:84861886200
VL - 32
SP - 1627
EP - 1631
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
SN - 0928-4931
IS - 6
ER -