Blockage of Nerve Growth Factor Action in PC12h Cells by Staurosporine, a Potent Protein Kinase Inhibitor

Seiichi Hashimoto, Akihiko Hagino

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Abstract: Staurosporine, which has a structure similar to that of K‐252a, a potent protein kinase inhibitor that blocks nerve growth factor (NGF) action in PC12 and PC12h cells, is also known as a potent inhibitor of several protein kinases. This study shows that in PC12h cells staurosporine has a dual action: at lower concentrations than that required by K‐252a, it is an inhibitor of NGF induction of neurite formation and of changes in the phosphorylation of specific proteins, whereasat concentrations higher than that required to inhibit NGF‐induced neurite outgrowth, it rapidly enhances outgrowth by itself.

Original languageEnglish
Pages (from-to)1675-1685
Number of pages11
JournalJournal of Neurochemistry
Volume53
Issue number6
DOIs
Publication statusPublished - 1989 Dec
Externally publishedYes

Keywords

  • Nerve growth factor
  • Neurite outgrowth
  • PC12h
  • Protein kinase inhibitor
  • Staurosporine

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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