Black-Tea Polyphenols Suppress Postprandial Hypertriacylglycerolemia by Suppressing Lymphatic Transport of Dietary Fat in Rats

Makoto Kubayashi, Masaki Ichitani, Yuko Suzuki, Tomonori Unno, Takashi Sugawara, Takashi Yamahira, Masaki Kato, Takanobu Takihara, Yuko Sagesaka, Takami Kakuda, Ikuo Ikeda

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

Administration of black-tea polyphenols (BTP) at 100 and 200 mg/kg of body weight in rats suppressed postprandial hypertriacylglycerolemia in a dose-dependent manner. Administration of BTP also suppressed lymphatic recovery of 14C-trioleoylglycerol in rats that were cannulated in the thoracic duct. BTP dose-dependently inhibited the activity of pancreatic lipase in vitro with an IC50 of 0.254 mg/mL. When purified theaflavins, which are components of BTP, were used, theaflavins with galloyl moieties, but not those without galloyl moiety, inhibited the activity of pancreatic lipase. Theaflavin-3,3′-digallate (TFDG) was more effective in inhibiting the activity of pancreatic lipase than epigallocatechin gallate (EGCG), epicatechin gallate (ECG), and a mixture of EGCG and ECG. BTP and TFDG had a similar effect in inhibiting the activity of pancreatic lipase when the total polyphenol amount was adjusted to the same. BTP had no effect on micellar solubility of hydrolysis products of triacylglycerol. These results suggest that BTP suppressed postprandial hypertriacylglycerolemia by reducing triacylglycerol absorption via the inhibition of pancreatic lipase activity.

Original languageEnglish
Pages (from-to)7131-7136
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume57
Issue number15
DOIs
Publication statusPublished - 2009 Aug 12

Keywords

  • Black-tea polyphenols
  • Pancreatic lipase
  • Postprandial hypertriacylglycerolemia
  • Rats
  • Theaflavins

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Fingerprint Dive into the research topics of 'Black-Tea Polyphenols Suppress Postprandial Hypertriacylglycerolemia by Suppressing Lymphatic Transport of Dietary Fat in Rats'. Together they form a unique fingerprint.

Cite this