Birth of mice produced by germ cell nuclear transfer

Hiromi Miki, Kimiko Inoue, Takashi Kohda, Arata Honda, Narumi Ogonuki, Misako Yuzuriha, Nathan Mise, Yasuhisa Matsui, Tadashi Baba, Kuniya Abe, Fumitoshi Ishino, Atsuo Ogura

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

That mammals can be cloned by nuclear transfer indicates that it is possible to reprogram the somatic cell genome to support full development. However, the developmental plasticity of germ cells is difficult to assess because genomic imprinting, which is essential for normal fetal development, is being reset at this stage. The anomalous influence of imprinting is corroborated by the poor development of mouse clones produced from primordial germ cells (PGCs) during imprinting erasure at embryonic day 11.5 or later. However, this can also be interpreted to mean that, unlike somatic cells, the genome of differentiated germ cells cannot be fully reprogrammed. We used younger PGCs (day 10.5) and eventually obtained four full-term fetuses. DNA methylation analyses showed that only embryos exhibiting normal imprinting developed to term. Thus, germ cell differentiation is not an insurmountable barrier to cloning, and imprinting status is more important than the origin of the nucleus donor cell per se as a determinant of developmental plasticity following nuclear transfer.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalGenesis
Volume41
Issue number2
DOIs
Publication statusPublished - 2005 Feb 1
Externally publishedYes

Keywords

  • Cloning
  • Genomic imprinting
  • Mouse
  • Nuclear transfer
  • Primordial germ cell

ASJC Scopus subject areas

  • Genetics

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