Biotin enhances ATP synthesis in pancreatic islets of the rat, resulting in reinforcement of glucose-induced insulin secretion

Hideyuki Sone, Yuka Sasaki, Michio Komai, Masaaki Toyomizu, Yasuo Kagawa, Yuji Furukawa

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Previous studies showed that biotin enhanced glucose-induced insulin secretion. Changes in the cytosolic ATP/ADP ratio in the pancreatic islets participate in the regulation of insulin secretion by glucose. In the present study we investigated whether biotin regulates the cytosolic ATP/ADP ratio in glucose-stimulated islets. When islets were stimulated with glucose plus biotin, the ATP/ADP ratio increased to approximately 160% of the ATP/ADP ratio in islets stimulated with glucose alone. The rate of glucose oxidation, assessed by CO2 production, was also about 2-fold higher in islets treated with biotin. These increasing effects of biotin were proportional to the effects seen in insulin secretion. There are no previous reports of vitamins, such as biotin, directly affecting ATP synthesis. Our data indicate that biotin enhances ATP synthesis in islets following the increased rate of substrate oxidation in mitochondria and that, as a consequence of these events, glucose-induced insulin release is reinforced by biotin.

Original languageEnglish
Pages (from-to)824-829
Number of pages6
JournalBiochemical and biophysical research communications
Volume314
Issue number3
DOIs
Publication statusPublished - 2004 Feb 13

Keywords

  • ATP/ADP
  • Biotin
  • Glucose oxidation
  • Insulin secretion

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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