Biosynthetic Study on Antihypercholesterolemic Agent Phomoidride: General Biogenesis of Fungal Dimeric Anhydrides

Ryuya Fujii; Yusuke Matsu; Atsushi Minami; Shota Nagamine; Ichiro Takeuchi; Katsuya Gomi; Hideaki Oikawa

doi:10.1021/acs.orglett.5b02934

Biosynthetic Study on Antihypercholesterolemic Agent Phomoidride: General Biogenesis of Fungal Dimeric Anhydrides

Ryuya Fujii, Yusuke Matsu, Atsushi Minami, Shota Nagamine, Ichiro Takeuchi, Katsuya Gomi, Hideaki Oikawa

AGR - Bioscience and Biotechnology for Future Bioindustries

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

To elucidate the general biosynthetic pathway of fungal dimeric anhydrides, a gene cluster for the biosynthesis of the antihy-percholesterolemic agent phomoidride was identified by heterologous expression of candidate genes encoding the highly reducing polyketide synthase, alkylcitrate synthase (ACS), and alkylcitrate dehydratase (ACDH). An in vitro analysis of ACS and ACDH revealed that they give rise to anhydride monomers. Based on the established monomer biosynthesis, we propose a general biogenesis of dimeric anhydrides involving a single donor unit and four acceptor units.

Original language	English
Pages (from-to)	5658-5661
Number of pages	4
Journal	Organic letters
Volume	17
Issue number	22
DOIs	https://doi.org/10.1021/acs.orglett.5b02934
Publication status	Published - 2015 Nov 20

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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title = "Biosynthetic Study on Antihypercholesterolemic Agent Phomoidride: General Biogenesis of Fungal Dimeric Anhydrides",

abstract = "To elucidate the general biosynthetic pathway of fungal dimeric anhydrides, a gene cluster for the biosynthesis of the antihy-percholesterolemic agent phomoidride was identified by heterologous expression of candidate genes encoding the highly reducing polyketide synthase, alkylcitrate synthase (ACS), and alkylcitrate dehydratase (ACDH). An in vitro analysis of ACS and ACDH revealed that they give rise to anhydride monomers. Based on the established monomer biosynthesis, we propose a general biogenesis of dimeric anhydrides involving a single donor unit and four acceptor units.",

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T1 - Biosynthetic Study on Antihypercholesterolemic Agent Phomoidride

T2 - General Biogenesis of Fungal Dimeric Anhydrides

AU - Fujii, Ryuya

AU - Matsu, Yusuke

AU - Minami, Atsushi

AU - Nagamine, Shota

AU - Takeuchi, Ichiro

AU - Gomi, Katsuya

AU - Oikawa, Hideaki

PY - 2015/11/20

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AB - To elucidate the general biosynthetic pathway of fungal dimeric anhydrides, a gene cluster for the biosynthesis of the antihy-percholesterolemic agent phomoidride was identified by heterologous expression of candidate genes encoding the highly reducing polyketide synthase, alkylcitrate synthase (ACS), and alkylcitrate dehydratase (ACDH). An in vitro analysis of ACS and ACDH revealed that they give rise to anhydride monomers. Based on the established monomer biosynthesis, we propose a general biogenesis of dimeric anhydrides involving a single donor unit and four acceptor units.

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