Biosynthesis of various types of collagen by human hepatoma cells in vitro

Ryojin Uchino; Takahiko Nohara; Eiichi Okamoto; Manabu Fukumoto; Osamu Midorikawa

doi:10.1007/BF02890131

Biosynthesis of various types of collagen by human hepatoma cells in vitro

Ryojin Uchino, Takahiko Nohara, Eiichi Okamoto, Manabu Fukumoto, Osamu Midorikawa

Division of Cancer Science

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A cloned human hepatoma cell line (HH2-1) produced and formed collagen fibers in vitro. The relative rate of collagen synthesis by the cells was increased with an enhancement of the cell density. An analysis of the components of the collagen using sodium dodecyl sulfatepolyacrylamide gel electrophoresis revealed that the cells synthesized interstitial collagen, types I and III, and other collagenous proteins. Thus, human hepatoma cells may play an important role in the formation of stromal collagen in the tumor.

Original language	English
Pages (from-to)	229-236
Number of pages	8
Journal	Virchows Archiv B Cell Pathology Including Molecular Pathology
Volume	48
Issue number	1
DOIs	https://doi.org/10.1007/BF02890131
Publication status	Published - 1985 Jan 1

Keywords

Collagen
Hepatoma
Liver cell carcinoma

ASJC Scopus subject areas

Pathology and Forensic Medicine

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AU - Uchino, Ryojin

AU - Nohara, Takahiko

AU - Okamoto, Eiichi

AU - Fukumoto, Manabu

AU - Midorikawa, Osamu

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N2 - A cloned human hepatoma cell line (HH2-1) produced and formed collagen fibers in vitro. The relative rate of collagen synthesis by the cells was increased with an enhancement of the cell density. An analysis of the components of the collagen using sodium dodecyl sulfatepolyacrylamide gel electrophoresis revealed that the cells synthesized interstitial collagen, types I and III, and other collagenous proteins. Thus, human hepatoma cells may play an important role in the formation of stromal collagen in the tumor.

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KW - Liver cell carcinoma

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