Biomimetic microfluidic device for in vitro antihypertensive drug evaluation

Lei Li, Xiaoqing Lv, Serge Ostrovidov, Xuetao Shi, Ning Zhang, Jing Liu

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)

    Abstract

    Microfluidic devices have emerged as revolutionary, novel platforms for in vitro drug evaluation. In this work, we developed a facile method for evaluating antihypertensive drugs using a microfluidic chip. This microfluidic chip was generated using the elastic material poly(dimethylsiloxane) (PDMS) and a microchannel structure that simulated a blood vessel as fabricated on the chip. We then cultured human umbilical vein endothelial cells (HUVECs) inside the channel. Different pressures and shear stresses could be applied on the cells. The generated vessel mimics can be used for evaluating the safety and effects of antihypertensive drugs. Here, we used hydralazine hydrochloride as a model drug. The results indicated that hydralazine hydrochloride effectively decreased the pressure-induced dysfunction of endothelial cells. This work demonstrates that our microfluidic system provides a convenient and cost-effective platform for studying cellular responses to drugs under mechanical pressure.

    Original languageEnglish
    Pages (from-to)2009-2015
    Number of pages7
    JournalMolecular pharmaceutics
    Volume11
    Issue number7
    DOIs
    Publication statusPublished - 2014 Jul 7

    Keywords

    • antihypertensive drug evaluation
    • endothelial cells
    • mechanical pressure
    • microfluidic chip

    ASJC Scopus subject areas

    • Molecular Medicine
    • Pharmaceutical Science
    • Drug Discovery

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