Biomimetic apatite deposition on polymeric microspheres treated with a calcium silicate solution

Isabel B. Leonor, Francisco Balas, Masakazu Kawashita, Rui L. Reis, Tadashi Kokubo, Takashi Nakamura

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Bioactive polymeric microspheres can be prepared by means of coating them with a calcium silicate solution and subsequently soaking in a simulated body fluid (SBF). Such combination should allow for the development of bioactive microspheres for several applications in the medical field including tissue engineering carriers. Four types of polymeric microspheres, with different sizes, were used in this work: (i) ethylene-vinyl alcohol copolymer (20-30 μm), (ii) polyamide 12 with 10% magnetite (100 μm), (iii) polyamide 12 (10-30 μm) and (iv) polyamide 12 (300 μm). These microspheres were soaked in a calcium silicate solution at 36.5°C for various periods of time under different conditions. Afterwards, they were dried in air at 60 and 100°C for 24 hr. Then, the samples were soaked in SBF for 1, 3, and 7 days. Fourier transformed infrared spectroscopy, thin-film X-ray diffraction, and scanning electron microscopy showed that after the calcium silicate treatment and the subsequent soaking in SBF, the microspheres successfully formed an apatite layer on their surfaces in SBF within 7 days due to the formation of silanol groups, which are effective for apatite formation.

Original languageEnglish
Pages (from-to)239-247
Number of pages9
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Issue number1
Publication statusPublished - 2009 Oct
Externally publishedYes


  • Biomimetics
  • Calcium silicate
  • Polymeric microspheres
  • Silanol (Si-OH)
  • Sol-gel processes

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering


Dive into the research topics of 'Biomimetic apatite deposition on polymeric microspheres treated with a calcium silicate solution'. Together they form a unique fingerprint.

Cite this