Biomechanics of C2C12 Cells Observed with Cellular Resolution Scanning Acoustic Microscope Combined with Optical Microscope

Ryo Hirano, Makoto Kanzaki, Mototaka Arakawa, Norma Hermawan, Kazuto Kobayashi, Yoshifumi Saijo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Biomechanics of the cell indicates the inner structure and viability of the cell. Mechanical properties are represented by acoustic properties such as speed of sound (SOS) or acoustic impedance. In the present study, cellular resolution scanning acoustic microscope combined with optical microscope (OptSAM) is developed to observe the change of mechanical properties in cell differentiation. Main part of the OptSAM was consisted of 350 MHz ultrasound transducer mechanically scanned by a piezo-actuator. Thickness, SOS, acoustic impedance, density and elastic bulk modulus of the cell were deduced by the ultrasound responses in both time domain and frequency domain. C2C12 cell changing its form from myoblast to myotube was observed by OptSAM. The value of bulk modulus slightly increased in response to differentiation process. OptSAM non-invasively provides important information on biomechanics of cells without contact or staining.

Original languageEnglish
Title of host publication2019 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages4828-4831
Number of pages4
ISBN (Electronic)9781538613115
DOIs
Publication statusPublished - 2019 Jul
Event41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019 - Berlin, Germany
Duration: 2019 Jul 232019 Jul 27

Publication series

NameProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN (Print)1557-170X

Conference

Conference41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019
CountryGermany
CityBerlin
Period19/7/2319/7/27

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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