Biomechanics of an isolated single stress fiber

Masaaki Sato, Shinji Deguchi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Knowledge of mechanical properties of stress fiber (SF), a bundle of actin filaments, is crucial for understanding its role in mechanotransduction in adherent cells. Here, we characterized tensile properties of single SFs by in vitro manipulation. SFs were isolated from cultured vascular smooth muscle cells with a combination of low ionic-strength extraction and detergent extraction and were stretched until breaking. The breaking force of the SFs for stretching was, on average, 377 nN, which was greater than actin filaments, 600 pN. The Young’s modulus was estimated as 1.45 MPa, which was three orders of magnitude lower than actin filaments. Strain-induced hardening, a common mechanical behavior of living adherent cells, was observed in a physiological strain range of the force-strain curves. Estimated force level of physiological tension in single SFs was the same order of magnitude with that of the substrate traction force of adherent cells required for maintenance of cell integrity. These results suggest that SFs are a principal subcellular component in bearing intracellular stresses.

Original languageEnglish
Title of host publicationTributes to Yuan-Cheng Fung on his 90th Birthday
Subtitle of host publicationBiomechanics: From Molecules to Man
PublisherWorld Scientific Publishing Co.
Pages13-20
Number of pages8
ISBN (Electronic)9789814289955
ISBN (Print)9814289876, 9789814289870
DOIs
Publication statusPublished - 2009 Jan 1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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    Sato, M., & Deguchi, S. (2009). Biomechanics of an isolated single stress fiber. In Tributes to Yuan-Cheng Fung on his 90th Birthday: Biomechanics: From Molecules to Man (pp. 13-20). World Scientific Publishing Co.. https://doi.org/10.1142/9789814289955_0002