Biological significance of carbohydrate chains on monoclonal antibodies

M. Nose, H. Wigzell

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357 Citations (Scopus)

Abstract

We have prepared monoclonal hapten-specific mouse IgG2b antibodies depleted of asparagine-linked carbohydrate chains by treating the hybridoma cells with tunicamycin. The carbohydrate-deficient antibodies behaved in an identical manner to the normal antibodies wth regard to fine antigen-binding reactivity (a Fab fragment feature) and protein A binding capacity [a feature requiring integrity of the C(H)2 and C(H)3 domain-interaction regions in the constant region of the heavy chain (C(H))]. However, they lost the ability to activate complement, to bind to Fc receptors on macrophages, and to induce antibody-dependent cellular cytotoxicity. Furthermore, antigen-antibody complexes produced from such carbohydrate-deficient antibodies failed to be eliminated rapidly from the circulation. We conclude that removal of carbohydrate chains from IgG molecules may have a profound and highly select impact on the biological activity to these antibodies.

Original languageEnglish
Pages (from-to)6632-6636
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume80
Issue number21 I
DOIs
Publication statusPublished - 1983 Jan 1

ASJC Scopus subject areas

  • General

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