Biological activities of novel derivatives of DIF-1 isolated from Dictyostelium

Haruhisa Kikuchi, Shinya Ishiko, Yoshiteru Oshima, Naomi Gokan, Kohei Hosaka, Yuzuru Kubohara

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The differentiation-inducing factor-1 (DIF-1) is a lipophilic signal molecule (chlorinated alkylphenone) that induces stalk cell differentiation in the cellular slime mold Dictyostelium discoideum. In addition, DIF-1 and its derivatives have been shown to possess anti-leukemic activity and glucose consumption-promoting activity in vitro in mammalian cells. In this study, to assess the chemical structure-effect relationship of DIF-1, we synthesized eight derivatives of DIF-1 and investigated their stalk cell-inducing activity in Dictyostelium cells and pharmacological activities in mammalian cells. Of the derivatives, two amide derivatives of DIF-1, whose hydrophobic indexes are close to that of DIF-1, induced stalk cell differentiation as strongly as DIF-1 in Dictyostelium cells. It was also found that some derivatives suppressed cell growth in human K562 leukemia cells and promoted glucose consumption in mouse 3T3-L1 cells. These results give us valuable information as to the chemical structure-effect relationship of DIF-1.

Original languageEnglish
Pages (from-to)1012-1017
Number of pages6
JournalBiochemical and biophysical research communications
Volume377
Issue number3
DOIs
Publication statusPublished - 2008 Dec 19

Keywords

  • DIF-1
  • Dictyostelium
  • Glucose metabolism
  • Leukemia
  • Stalk cell differentiation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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