Bioengineering a 3D integumentary organ system from iPS cells using an in vivo transplantation model

Ryoji Takagi, Junko Ishimaru, Ayaka Sugawara, Koh Ei Toyoshima, Kentaro Ishida, Miho Ogawa, Kei Sakakibara, Kyosuke Asakawa, Akitoshi Kashiwakura, Masamitsu Oshima, Ryohei Minamide, Akio Sato, Toshihiro Yoshitake, Akira Takeda, Hiroshi Egusa, Takashi Tsuji

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The integumentary organ system is a complex system that plays important roles in waterproofing, cushioning, protecting deeper tissues, excreting waste, and thermoregulation. We developed a novel in vivo transplantation model designated as a clustering-dependent embryoid body transplantation method and generated a bioengineered three-dimensional (3D) integumentary organ system, including appendage organs such as hair follicles and sebaceous glands, from induced pluripotent stem cells. This bioengineered 3D integumentary organ system was fully functional following transplantation into nude mice and could be properly connected to surrounding host tissues, such as the epidermis, arrector pili muscles, and nerve fibers, without tumorigenesis. The bioengineered hair follicles in the 3D integumentary organ system also showed proper hair eruption and hair cycles, including the rearrangement of follicular stem cells and their niches. Potential applications of the 3D integumentary organ system include an in vitro assay system, an animal model alternative, and a bioengineered organ replacement therapy.

Original languageEnglish
Article numbere1500887
JournalScience Advances
Volume2
Issue number4
DOIs
Publication statusPublished - 2016 Apr

ASJC Scopus subject areas

  • General

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    Takagi, R., Ishimaru, J., Sugawara, A., Toyoshima, K. E., Ishida, K., Ogawa, M., Sakakibara, K., Asakawa, K., Kashiwakura, A., Oshima, M., Minamide, R., Sato, A., Yoshitake, T., Takeda, A., Egusa, H., & Tsuji, T. (2016). Bioengineering a 3D integumentary organ system from iPS cells using an in vivo transplantation model. Science Advances, 2(4), [e1500887]. https://doi.org/10.1126/sciadv.1500887