Biodegradation of γ-hexachlorocyclohexane by transgenic hairy root cultures of Cucurbita moschata that accumulate recombinant bacterial LinA

Yoshihiko Nanasato, Sayuri Namiki, Masao Oshima, Ryota Moriuchi, Ken ichi Konagaya, Nobuyasu Seike, Takashi Otani, Yuji Nagata, Masataka Tsuda, Yutaka Tabei

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    Key message: γ-HCH was successfully degraded using LinA-expressed transgenic hairy root cultures ofCucurbita moschata. Fusing an endoplasmic reticulum-targeting signal peptide to LinA was essential for stable accumulation in the hairy roots. Abstract: The pesticide γ-hexachlorocyclohexane (γ-HCH) is a persistent organic pollutant (POP) that raises public health and environmental pollution concerns worldwide. Although several isolates of γ-HCH-degrading bacteria are available, inoculating them directly into γ-HCH-contaminated soil is ineffective because of the bacterial survival rate. Cucurbita species incorporate significant amounts of POPs from soils compared with other plant species. Here, we describe a novel bioremediation strategy that combines the bacterial degradation of γ-HCH and the efficient uptake of γ-HCH by Cucurbita species. We produced transgenic hairy root cultures of Cucurbita moschata that expressed recombinant bacterial linA, isolated from the bacterium Sphingobium japonicum UT26. The LinA protein was accumulated stably in the hairy root cultures by fusing an endoplasmic reticulum (ER)-targeting signal peptide to LinA. Then, we demonstrated that the cultures degraded more than 90 % of γ-HCH (1 ppm) overnight and produced the γ-HCH metabolite 1,2,4-trichlorobenzene, indicating that LinA degraded γ-HCH. These results indicate that the gene linA has high potential for phytoremediation of environmental γ-HCH.

    Original languageEnglish
    Pages (from-to)1963-1974
    Number of pages12
    JournalPlant Cell Reports
    Volume35
    Issue number9
    DOIs
    Publication statusPublished - 2016 Sep 1

    Keywords

    • Biodegradation
    • Cucurbita moschata
    • Hairy root cultures
    • LinA
    • Phytoremediation
    • γ-Hexachlorocyclohexane (γ-HCH)

    ASJC Scopus subject areas

    • Agronomy and Crop Science
    • Plant Science

    Fingerprint Dive into the research topics of 'Biodegradation of γ-hexachlorocyclohexane by transgenic hairy root cultures of Cucurbita moschata that accumulate recombinant bacterial LinA'. Together they form a unique fingerprint.

    Cite this