Biodegradable main-chain phosphate-caged fluorescein polymers for the evaluation of enzymatic activity

Kazuo Tanaka, Narufumi Kitamura, Yoshiki Chujo

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We describe here the biodegradable polymers consisted of the tandemly linked phosphate-caged fluorescein in the main chains. The caged-fluorescein monomer with the phosphoramidite groups at both hydroxyl groups was synthesized, and the polycondensations were executed in the presence of the diol derivatives which were promised to be the reactivity-tunable linker. After oxidation and the alkaline treatment for the deprotection, less-emissive polymeric products were obtained. The resulting polymers can release fluorescein by the digestion with alkaline phosphatase. We demonstrated that the enzymatic activity of the cell lysate can be quantified from the increase of the magnitude of fluorescence emission with the synthetic polymers.

Original languageEnglish
Pages (from-to)6180-6184
Number of pages5
JournalMacromolecules
Volume43
Issue number14
DOIs
Publication statusPublished - 2010 Jul 27
Externally publishedYes

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Inorganic Chemistry
  • Materials Chemistry

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