Biochemical quantization by microfluidic droplets for the development of microbe counter

Katsuhiro Aritome, Yuto Takahata, Kenji Sakamoto, Kenichi Noda, Akio Kuroda, Tomohiro Ishikawa, Ryo Miyake, Yuji Murakami

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This paper describes a newly proposed Microbe Counter with Biochemical Quantization to improve a low repro-ducibility of a conventional ATP method for hygienic management. The counter consists of a mixer, a microfluidic droplet generator, and a detector. Biochemical quantization by microfluidic droplets includes a partitioning step of sample water into droplets consisting one or less microbe and a detection step of each droplets. The concept is statistically discussed to show required specification for the counter. Yeast cell quantization, biochemiluminescence on a chip, mixing and reaction in microfluidic droplets, and biochemiluminescence in microfluidic droplets were also demonstrated and discussed.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages1136-1138
Number of pages3
Publication statusPublished - 2010 Dec 1
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 2010 Oct 32010 Oct 7

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume2

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/10/310/10/7

Keywords

  • AT P measurement
  • Biochemiluminescence
  • Droplet
  • Luciferase
  • Microbe

ASJC Scopus subject areas

  • Control and Systems Engineering

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