Biochemical quantization by microfluidic droplets for the development of microbe counter

Katsuhiro Aritome; Yuto Takahata; Kenji Sakamoto; Kenichi Noda; Akio Kuroda; Tomohiro Ishikawa; Ryo Miyake; Yuji Murakami

Biochemical quantization by microfluidic droplets for the development of microbe counter

Katsuhiro Aritome, Yuto Takahata, Kenji Sakamoto, Kenichi Noda, Akio Kuroda, Tomohiro Ishikawa, Ryo Miyake, Yuji Murakami

Micro System Integration Center (μSIC)

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

This paper describes a newly proposed Microbe Counter with Biochemical Quantization to improve a low repro-ducibility of a conventional ATP method for hygienic management. The counter consists of a mixer, a microfluidic droplet generator, and a detector. Biochemical quantization by microfluidic droplets includes a partitioning step of sample water into droplets consisting one or less microbe and a detection step of each droplets. The concept is statistically discussed to show required specification for the counter. Yeast cell quantization, biochemiluminescence on a chip, mixing and reaction in microfluidic droplets, and biochemiluminescence in microfluidic droplets were also demonstrated and discussed.

Original language	English
Title of host publication	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages	1136-1138
Number of pages	3
Publication status	Published - 2010 Dec 1
Event	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands Duration: 2010 Oct 3 → 2010 Oct 7

Publication series

Name	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume	2

Other

Other	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Country	Netherlands
City	Groningen
Period	10/10/3 → 10/10/7

Keywords

AT P measurement
Biochemiluminescence
Droplet
Luciferase
Microbe

ASJC Scopus subject areas

Control and Systems Engineering

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Cite this

Aritome, K., Takahata, Y., Sakamoto, K., Noda, K., Kuroda, A., Ishikawa, T., Miyake, R., & Murakami, Y. (2010). Biochemical quantization by microfluidic droplets for the development of microbe counter. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 1136-1138). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 2).

Biochemical quantization by microfluidic droplets for the development of microbe counter. / Aritome, Katsuhiro; Takahata, Yuto; Sakamoto, Kenji; Noda, Kenichi; Kuroda, Akio; Ishikawa, Tomohiro; Miyake, Ryo; Murakami, Yuji.

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. p. 1136-1138 (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 2).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Aritome, K, Takahata, Y, Sakamoto, K, Noda, K, Kuroda, A, Ishikawa, T, Miyake, R & Murakami, Y 2010, Biochemical quantization by microfluidic droplets for the development of microbe counter. in 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, vol. 2, pp. 1136-1138, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, Netherlands, 10/10/3.

Aritome K, Takahata Y, Sakamoto K, Noda K, Kuroda A, Ishikawa T et al. Biochemical quantization by microfluidic droplets for the development of microbe counter. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. p. 1136-1138. (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010).

Aritome, Katsuhiro ; Takahata, Yuto ; Sakamoto, Kenji ; Noda, Kenichi ; Kuroda, Akio ; Ishikawa, Tomohiro ; Miyake, Ryo ; Murakami, Yuji. / Biochemical quantization by microfluidic droplets for the development of microbe counter. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. pp. 1136-1138 (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010).

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abstract = "This paper describes a newly proposed Microbe Counter with Biochemical Quantization to improve a low repro-ducibility of a conventional ATP method for hygienic management. The counter consists of a mixer, a microfluidic droplet generator, and a detector. Biochemical quantization by microfluidic droplets includes a partitioning step of sample water into droplets consisting one or less microbe and a detection step of each droplets. The concept is statistically discussed to show required specification for the counter. Yeast cell quantization, biochemiluminescence on a chip, mixing and reaction in microfluidic droplets, and biochemiluminescence in microfluidic droplets were also demonstrated and discussed.",

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AU - Aritome, Katsuhiro

AU - Takahata, Yuto

AU - Sakamoto, Kenji

AU - Noda, Kenichi

AU - Kuroda, Akio

AU - Ishikawa, Tomohiro

AU - Miyake, Ryo

AU - Murakami, Yuji

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N2 - This paper describes a newly proposed Microbe Counter with Biochemical Quantization to improve a low repro-ducibility of a conventional ATP method for hygienic management. The counter consists of a mixer, a microfluidic droplet generator, and a detector. Biochemical quantization by microfluidic droplets includes a partitioning step of sample water into droplets consisting one or less microbe and a detection step of each droplets. The concept is statistically discussed to show required specification for the counter. Yeast cell quantization, biochemiluminescence on a chip, mixing and reaction in microfluidic droplets, and biochemiluminescence in microfluidic droplets were also demonstrated and discussed.

AB - This paper describes a newly proposed Microbe Counter with Biochemical Quantization to improve a low repro-ducibility of a conventional ATP method for hygienic management. The counter consists of a mixer, a microfluidic droplet generator, and a detector. Biochemical quantization by microfluidic droplets includes a partitioning step of sample water into droplets consisting one or less microbe and a detection step of each droplets. The concept is statistically discussed to show required specification for the counter. Yeast cell quantization, biochemiluminescence on a chip, mixing and reaction in microfluidic droplets, and biochemiluminescence in microfluidic droplets were also demonstrated and discussed.

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KW - Biochemiluminescence

KW - Droplet

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