TY - GEN
T1 - Biochemical quantization by microfluidic droplets for the development of microbe counter
AU - Aritome, Katsuhiro
AU - Takahata, Yuto
AU - Sakamoto, Kenji
AU - Noda, Kenichi
AU - Kuroda, Akio
AU - Ishikawa, Tomohiro
AU - Miyake, Ryo
AU - Murakami, Yuji
PY - 2010
Y1 - 2010
N2 - This paper describes a newly proposed Microbe Counter with Biochemical Quantization to improve a low repro-ducibility of a conventional ATP method for hygienic management. The counter consists of a mixer, a microfluidic droplet generator, and a detector. Biochemical quantization by microfluidic droplets includes a partitioning step of sample water into droplets consisting one or less microbe and a detection step of each droplets. The concept is statistically discussed to show required specification for the counter. Yeast cell quantization, biochemiluminescence on a chip, mixing and reaction in microfluidic droplets, and biochemiluminescence in microfluidic droplets were also demonstrated and discussed.
AB - This paper describes a newly proposed Microbe Counter with Biochemical Quantization to improve a low repro-ducibility of a conventional ATP method for hygienic management. The counter consists of a mixer, a microfluidic droplet generator, and a detector. Biochemical quantization by microfluidic droplets includes a partitioning step of sample water into droplets consisting one or less microbe and a detection step of each droplets. The concept is statistically discussed to show required specification for the counter. Yeast cell quantization, biochemiluminescence on a chip, mixing and reaction in microfluidic droplets, and biochemiluminescence in microfluidic droplets were also demonstrated and discussed.
KW - AT P measurement
KW - Biochemiluminescence
KW - Droplet
KW - Luciferase
KW - Microbe
UR - http://www.scopus.com/inward/record.url?scp=84884395156&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884395156&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84884395156
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 1136
EP - 1138
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -