Biochemical characteristics of the novel haloalkane dehalogenase DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58

Khomaini Hasan, Andrea Fortova, Tana Koudelakova, Radka Chaloupkova, Mayuko Ishitsuka, Yuji Nagata, Jiri Damborsky, Zbynek Prokop

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

We report the biochemical characterization of a novel haloalkane dehalogenase, DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilizing residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity toward racemic mixtures of chiral brominated alkanes and esters.

Original languageEnglish
Pages (from-to)1881-1884
Number of pages4
JournalApplied and environmental microbiology
Volume77
Issue number5
DOIs
Publication statusPublished - 2011 Mar
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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