Biochemical characteristics of the novel haloalkane dehalogenase DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58

Khomaini Hasan, Andrea Fortova, Tana Koudelakova, Radka Chaloupkova, Mayuko Ishitsuka, Yuji Nagata, Jiri Damborsky, Zbynek Prokop

    Research output: Contribution to journalArticlepeer-review

    28 Citations (Scopus)

    Abstract

    We report the biochemical characterization of a novel haloalkane dehalogenase, DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilizing residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity toward racemic mixtures of chiral brominated alkanes and esters.

    Original languageEnglish
    Pages (from-to)1881-1884
    Number of pages4
    JournalApplied and environmental microbiology
    Volume77
    Issue number5
    DOIs
    Publication statusPublished - 2011 Mar

    ASJC Scopus subject areas

    • Biotechnology
    • Food Science
    • Applied Microbiology and Biotechnology
    • Ecology

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