Biochemical Assay of G Protein-Coupled Receptor Oligomerization. Adenosine A1 and Thromboxane A2 Receptors Form the Novel Functional Hetero-oligomer

Natsumi Mizuno, Tokiko Suzuki, Yu Kishimoto, Noriyasu Hirasawa

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)


G protein-coupled receptors (GPCRs) are classified into a family of seven transmembrane receptors. Receptor oligomerization may be the key to the expression and function of these receptors, for example, ligand binding, desensitization, membrane trafficking, and signaling. The accumulation of evidence that GPCRs form an oligomerization with a functional alternation may change the strategy for the discovery of novel drugs targeting GPCRs. Identification of the oligomer is essential to elucidate GPCR oligomerization. GPCR oligomerizations have been demonstrated using various biochemical approaches, which include the coimmunoprecipitation method, fluorescence resonance energy transfer assay, and bioluminescence RET assay. Thus, various assays are useful for the study of GPCR oligomerization, and we should choose the best method to match the purpose. We previously targeted adenosine A1 and thromboxane A2 (TP) receptors to form a functionally novel hetero-oligomer, since both receptors function in the same cells. This chapter describes the methods used to detect GPCR oligomerization and alterations in the signaling pathways, principally according to our findings on oligomerization between adenosine A1 and TPα receptors.

Original languageEnglish
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
Number of pages15
Publication statusPublished - 2013

Publication series

NameMethods in Cell Biology
ISSN (Print)0091-679X


  • BRET
  • Coimmunoprecipitation
  • GPCR
  • Oligomerization
  • TPα receptor

ASJC Scopus subject areas

  • Cell Biology

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