Biochemical analysis of oligomerization of expanded polyalanine repeat proteins

Jun Nojima, Yoko Oma, Eugene Futai, Noboru Sasagawa, Reiko Kuroda, Boris Turk, Shoichi Ishiura

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Many human proteins contain amino acid repeats that can form homopolymeric amino acid (HPAA) tracts. HPAA tract proteins that contain polyalanine sequences promote diseases, including oculopharyngeal muscular dystrophy. The pathological properties of these proteins develop when the repeats match or exceed ∼20 residues. We analyzed the oligomerization of yellow fluorescent protein (YFP) and GST fusion proteins containing >20 alanine repeats by using sucrose density gradient centrifugation. YFP and GST fusion proteins having 23 polyalanine residues sedimented readily in sucrose density gradients, suggesting instability and oligomerization of proteins with an excess of 20 alanine repeats. Moreover, GST fusion proteins were resistant to trypsin digestion after oligomerization. Oligomerized artificial proteins with long polyalanine repeats may be suitable models for studying polyalanine-related diseases.

Original languageEnglish
Pages (from-to)2290-2296
Number of pages7
JournalJournal of Neuroscience Research
Volume87
Issue number10
DOIs
Publication statusPublished - 2009 Aug 1
Externally publishedYes

Keywords

  • Oligomerization
  • Polyalanine diseases
  • Sucrose density centrifugation
  • Trypsin digestion

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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