TY - GEN
T1 - Bio-imaging by functional nano-particles of nano to macro scale
AU - Takeda, M.
AU - Tada, H.
AU - Kawai, M.
AU - Sakurai, Y.
AU - Higuchi, H.
AU - Gonda, K.
AU - Ishida, T.
AU - Ohuchi, Noriaki
PY - 2009
Y1 - 2009
N2 - Nano-materials are expected to be used for medical applications such as research on pharmacokinetics and novel therapeutic agents. Their acute toxicity should be examined and systemic distribution of the nano-materials should also be estimated. We established a high resolution in vivo 3D microscopic system for a novel fluorescence imaging method at single molecular level. We measured in vivo movement of CdSe nano-particles (quantum dots (QDs)) conjugated with monoclonal anti-HER2 antibody (Trastuzumab) in tumor vessel to breast cancer cells. The HER2 protein expressed in cancer cells and its in vivo dynamics were visualized by QDs at nano-scale. It suggests future utilization of the system to improve drug delivery system to target the primary and metastatic tumors for made to order treatment. We also describe a novel contrast medium for X-ray and its distribution in organs. Nano-sized silica coated silver iodide beads exhibited contrast enhancementin the tumor for over one hour. Sentinel node navigation is one of the important clinical applications for fluorescence nano-particles and silver iodide beads. Our experimental data of those nano-particles in experimental model have shown the potential of fluorescence and X-ray measurement to be alternatives to existing tracers. Future innovation in cancer imaging by nano-technology and novel measurement technology will provide great improvement, not only in clinical field of prevention, diagnosis and treatment of human disease, but also in basic medical science.
AB - Nano-materials are expected to be used for medical applications such as research on pharmacokinetics and novel therapeutic agents. Their acute toxicity should be examined and systemic distribution of the nano-materials should also be estimated. We established a high resolution in vivo 3D microscopic system for a novel fluorescence imaging method at single molecular level. We measured in vivo movement of CdSe nano-particles (quantum dots (QDs)) conjugated with monoclonal anti-HER2 antibody (Trastuzumab) in tumor vessel to breast cancer cells. The HER2 protein expressed in cancer cells and its in vivo dynamics were visualized by QDs at nano-scale. It suggests future utilization of the system to improve drug delivery system to target the primary and metastatic tumors for made to order treatment. We also describe a novel contrast medium for X-ray and its distribution in organs. Nano-sized silica coated silver iodide beads exhibited contrast enhancementin the tumor for over one hour. Sentinel node navigation is one of the important clinical applications for fluorescence nano-particles and silver iodide beads. Our experimental data of those nano-particles in experimental model have shown the potential of fluorescence and X-ray measurement to be alternatives to existing tracers. Future innovation in cancer imaging by nano-technology and novel measurement technology will provide great improvement, not only in clinical field of prevention, diagnosis and treatment of human disease, but also in basic medical science.
KW - HER2
KW - Single molecular imaging
KW - nano-technology
KW - quantum dots
KW - silica coated silver iodide beads
UR - http://www.scopus.com/inward/record.url?scp=84891955450&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84891955450&partnerID=8YFLogxK
U2 - 10.1007/978-3-540-92841-6_570
DO - 10.1007/978-3-540-92841-6_570
M3 - Conference contribution
AN - SCOPUS:84891955450
SN - 9783540928409
T3 - IFMBE Proceedings
SP - 2272
EP - 2274
BT - 13th International Conference on Biomedical Engineering - ICBME 2008
T2 - 13th International Conference on Biomedical Engineering, ICBME 2008
Y2 - 3 December 2008 through 6 December 2008
ER -
