Binding sites for melanin-concentrating hormone in the human brain

Masahiko Sone, Kazuhiro Takahashi, Osamu Murakami, Kazuhito Totsune, Zenei Arihara, Fumitoshi Satoh, Hironobu Sasano, Hisao Ito, Toraichi Mouri

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Binding sites for melanin-concentrating hormone (MCH) in human brain were investigated and characterized by radioligand binding. Specific binding sites for MCH were present in every region of human brain (cerebral cortex, cerebellum, thalamus, hypothalamus, pons, and medulla oblongata) obtained at autopsy. α-Melanocyte stimulating hormone or ACTH was a poor inhibitor of 125I-MCH binding (IC50 > 1 μM) compared with MCH (IC50 = 0.3 ± 0.07 nM, mean ± SEM, n = 3). Scatchard plots of 125I-MCH binding in human brain (thalamus) gave a dissociation constant of 0.2 ± 0.06 nM and maximal binding of 5.8 ± 0.3 fmol/mg protein (n = 3). These findings suggest that specific MCH binding sites that differ from the melanocortin receptors exist in human brain. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)245-250
Number of pages6
JournalPeptides
Volume21
Issue number2
DOIs
Publication statusPublished - 2000 Feb

Keywords

  • Human brain
  • Melanin-concentrating hormone
  • Melanocortin
  • Receptor

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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