Binding of the origin of replication of Escherichia coli to the outer membrane

William G. Hendrickson; Tomonobu Kusano; Hiroshi Yamaki; Ramaswamy Balakrishnan; Mary King; Jennifer Murchie; Moselio Schaechter

doi:10.1016/0092-8674(82)90296-3

Binding of the origin of replication of Escherichia coli to the outer membrane

William G. Hendrickson, Tomonobu Kusano, Hiroshi Yamaki, Ramaswamy Balakrishnan, Mary King, Jennifer Murchie, Moselio Schaechter

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

The replication origin of the Escherichia coli chromosome binds with high affinity to outer membrane preparations. This binding requires a 460 bp stretch of origin DNA between positions -40 and 420 of the oriC map. Specific binding can be detected by the use of a membrane filter retention assay in the presence of excess calf thymus DNA. This binding is enhanced by divalent cations and takes place specifically at a few (0.7-3.0) membrane sites per cell. The apparent affinity of origin DNA for membranes is enhanced by two peptides, (55 kilodaltons (kd) and 75 kd), which remain attached to the DNA through treatment with 5.5 M cesium chloride.

Original language	English
Pages (from-to)	915-923
Number of pages	9
Journal	Cell
Volume	30
Issue number	3
DOIs	https://doi.org/10.1016/0092-8674(82)90296-3
Publication status	Published - 1982 Oct

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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title = "Binding of the origin of replication of Escherichia coli to the outer membrane",

abstract = "The replication origin of the Escherichia coli chromosome binds with high affinity to outer membrane preparations. This binding requires a 460 bp stretch of origin DNA between positions -40 and 420 of the oriC map. Specific binding can be detected by the use of a membrane filter retention assay in the presence of excess calf thymus DNA. This binding is enhanced by divalent cations and takes place specifically at a few (0.7-3.0) membrane sites per cell. The apparent affinity of origin DNA for membranes is enhanced by two peptides, (55 kilodaltons (kd) and 75 kd), which remain attached to the DNA through treatment with 5.5 M cesium chloride.",

author = "Hendrickson, {William G.} and Tomonobu Kusano and Hiroshi Yamaki and Ramaswamy Balakrishnan and Mary King and Jennifer Murchie and Moselio Schaechter",

year = "1982",

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T1 - Binding of the origin of replication of Escherichia coli to the outer membrane

AU - Hendrickson, William G.

AU - Kusano, Tomonobu

AU - Yamaki, Hiroshi

AU - Balakrishnan, Ramaswamy

AU - King, Mary

AU - Murchie, Jennifer

AU - Schaechter, Moselio

PY - 1982/10

Y1 - 1982/10

N2 - The replication origin of the Escherichia coli chromosome binds with high affinity to outer membrane preparations. This binding requires a 460 bp stretch of origin DNA between positions -40 and 420 of the oriC map. Specific binding can be detected by the use of a membrane filter retention assay in the presence of excess calf thymus DNA. This binding is enhanced by divalent cations and takes place specifically at a few (0.7-3.0) membrane sites per cell. The apparent affinity of origin DNA for membranes is enhanced by two peptides, (55 kilodaltons (kd) and 75 kd), which remain attached to the DNA through treatment with 5.5 M cesium chloride.

AB - The replication origin of the Escherichia coli chromosome binds with high affinity to outer membrane preparations. This binding requires a 460 bp stretch of origin DNA between positions -40 and 420 of the oriC map. Specific binding can be detected by the use of a membrane filter retention assay in the presence of excess calf thymus DNA. This binding is enhanced by divalent cations and takes place specifically at a few (0.7-3.0) membrane sites per cell. The apparent affinity of origin DNA for membranes is enhanced by two peptides, (55 kilodaltons (kd) and 75 kd), which remain attached to the DNA through treatment with 5.5 M cesium chloride.

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