TY - JOUR
T1 - Binding of the origin of replication of Escherichia coli to the outer membrane
AU - Hendrickson, William G.
AU - Kusano, Tomonobu
AU - Yamaki, Hiroshi
AU - Balakrishnan, Ramaswamy
AU - King, Mary
AU - Murchie, Jennifer
AU - Schaechter, Moselio
N1 - Funding Information:
This work was supported by a grant from the National Institutes of Health.
PY - 1982/10
Y1 - 1982/10
N2 - The replication origin of the Escherichia coli chromosome binds with high affinity to outer membrane preparations. This binding requires a 460 bp stretch of origin DNA between positions -40 and 420 of the oriC map. Specific binding can be detected by the use of a membrane filter retention assay in the presence of excess calf thymus DNA. This binding is enhanced by divalent cations and takes place specifically at a few (0.7-3.0) membrane sites per cell. The apparent affinity of origin DNA for membranes is enhanced by two peptides, (55 kilodaltons (kd) and 75 kd), which remain attached to the DNA through treatment with 5.5 M cesium chloride.
AB - The replication origin of the Escherichia coli chromosome binds with high affinity to outer membrane preparations. This binding requires a 460 bp stretch of origin DNA between positions -40 and 420 of the oriC map. Specific binding can be detected by the use of a membrane filter retention assay in the presence of excess calf thymus DNA. This binding is enhanced by divalent cations and takes place specifically at a few (0.7-3.0) membrane sites per cell. The apparent affinity of origin DNA for membranes is enhanced by two peptides, (55 kilodaltons (kd) and 75 kd), which remain attached to the DNA through treatment with 5.5 M cesium chloride.
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U2 - 10.1016/0092-8674(82)90296-3
DO - 10.1016/0092-8674(82)90296-3
M3 - Article
C2 - 6754093
AN - SCOPUS:0020362150
VL - 30
SP - 915
EP - 923
JO - Cell
JF - Cell
SN - 0092-8674
IS - 3
ER -