Binding of metal ions by pyrimidine base pairs in DNA duplexes

Akira Ono, Hidetaka Torigoe, Yoshiyuki Tanaka, Itaru Okamoto

Research output: Contribution to journalReview articlepeer-review

233 Citations (Scopus)

Abstract

Pyrimidine base pairs in DNA duplexes selectively capture metal ions to form metal ion-mediated base pairs, which can be evaluated by thermal denaturation, isothermal titration calorimetry, and nuclear magnetic resonance spectroscopy. In this critical review, we discuss the metal ion binding of pyrimidine bases (thymine, cytosine, 4-thiothymine, 2-thiothymine, 5-fluorouracil) in DNA duplexes. Thymine-thymine (T-T) and cytosine-cytosine (C-C) base pairs selectively capture Hg(ii) and Ag(i) ions, respectively, and the metallo-base pairs, T-Hg(ii)-T and C-Ag(i)-C, are formed in DNA duplexes. The metal ion binding properties of the pyrimidine-pyrimidine pairs can be changed by small chemical modifications. The binding selectivity of a metal ion to a 5-fluorouracil-5-fluorouracil pair in a DNA duplex can be switched by changing the pH of the solution. Two silver ions bind to each thiopyrimidine-thiopyrimidine pair in the duplexes, and the duplexes are largely stabilized. Oligonucleotides containing these bases are commercially available and can readily be applied in many scientific fields (86 references).

Original languageEnglish
Pages (from-to)5855-5866
Number of pages12
JournalChemical Society Reviews
Volume40
Issue number12
DOIs
Publication statusPublished - 2011 Dec 1

ASJC Scopus subject areas

  • Chemistry(all)

Fingerprint

Dive into the research topics of 'Binding of metal ions by pyrimidine base pairs in DNA duplexes'. Together they form a unique fingerprint.

Cite this