TY - JOUR
T1 - Binding of metal ions by pyrimidine base pairs in DNA duplexes
AU - Ono, Akira
AU - Torigoe, Hidetaka
AU - Tanaka, Yoshiyuki
AU - Okamoto, Itaru
PY - 2011/12/1
Y1 - 2011/12/1
N2 - Pyrimidine base pairs in DNA duplexes selectively capture metal ions to form metal ion-mediated base pairs, which can be evaluated by thermal denaturation, isothermal titration calorimetry, and nuclear magnetic resonance spectroscopy. In this critical review, we discuss the metal ion binding of pyrimidine bases (thymine, cytosine, 4-thiothymine, 2-thiothymine, 5-fluorouracil) in DNA duplexes. Thymine-thymine (T-T) and cytosine-cytosine (C-C) base pairs selectively capture Hg(ii) and Ag(i) ions, respectively, and the metallo-base pairs, T-Hg(ii)-T and C-Ag(i)-C, are formed in DNA duplexes. The metal ion binding properties of the pyrimidine-pyrimidine pairs can be changed by small chemical modifications. The binding selectivity of a metal ion to a 5-fluorouracil-5-fluorouracil pair in a DNA duplex can be switched by changing the pH of the solution. Two silver ions bind to each thiopyrimidine-thiopyrimidine pair in the duplexes, and the duplexes are largely stabilized. Oligonucleotides containing these bases are commercially available and can readily be applied in many scientific fields (86 references).
AB - Pyrimidine base pairs in DNA duplexes selectively capture metal ions to form metal ion-mediated base pairs, which can be evaluated by thermal denaturation, isothermal titration calorimetry, and nuclear magnetic resonance spectroscopy. In this critical review, we discuss the metal ion binding of pyrimidine bases (thymine, cytosine, 4-thiothymine, 2-thiothymine, 5-fluorouracil) in DNA duplexes. Thymine-thymine (T-T) and cytosine-cytosine (C-C) base pairs selectively capture Hg(ii) and Ag(i) ions, respectively, and the metallo-base pairs, T-Hg(ii)-T and C-Ag(i)-C, are formed in DNA duplexes. The metal ion binding properties of the pyrimidine-pyrimidine pairs can be changed by small chemical modifications. The binding selectivity of a metal ion to a 5-fluorouracil-5-fluorouracil pair in a DNA duplex can be switched by changing the pH of the solution. Two silver ions bind to each thiopyrimidine-thiopyrimidine pair in the duplexes, and the duplexes are largely stabilized. Oligonucleotides containing these bases are commercially available and can readily be applied in many scientific fields (86 references).
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U2 - 10.1039/c1cs15149e
DO - 10.1039/c1cs15149e
M3 - Review article
C2 - 21826352
AN - SCOPUS:81455127305
VL - 40
SP - 5855
EP - 5866
JO - Chemical Society Reviews
JF - Chemical Society Reviews
SN - 0306-0012
IS - 12
ER -