Binding of macrophage colony-stimulating factor to serum proteins

T. Ohtsuki, K. Hatake, M. Ikeda, M. Yamada, K. Motoyoshi, Y. Miura

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Although three molecular forms of macrophage colony-stimulating factor (M-CSF) have been reported, Western blot analysis of immunoaffinity-purified M-CSF from human blood has shown that the major species of M-CSF in the serum has a molecular weight (MW) of 85 kD. Superose-12 gel filtration chromatography of immunoaffinity-purified serum M-CSF showed the presence of M-CSF-positive fraction in a higher MW area compared with the elution profile of recombinant human (rh) 85-kD M-CSF. Western blot analysis of the higher MW fraction showed that the M-CSF was the same as rh 85-kD M-CSF (not proteoglycan form of M-CSF), indicating that, in the serum, M-CSF exists bound to some serum proteins. To detect the serum proteins, we performed M-CSF-bound column chromatography. The eluate contained at least three serum proteins including albumin and IgG. This result was supported by chromatography using biotinylated M-CSF and avidin-agarose. The binding between rhM-CSF and albumin or IgG was also demonstrated by high-performance liquid chromatography (HPLC) fractionation of the mixture and enzyme-linked immunosorbent assay (ELISA) of the fractions. In the serum, a fraction of M-CSF seems to be complexed with serum proteins such as albumin and IgG.

Original languageEnglish
Pages (from-to)101-107
Number of pages7
JournalExperimental Hematology
Volume24
Issue number2
Publication statusPublished - 1996 Mar 20
Externally publishedYes

Keywords

  • IgG
  • M-CSF
  • albumin
  • carrier
  • serum

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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