Binding of GID1 to DELLAs promotes dissociation of GAF1 from DELLA in GA dependent manner

Jutarou Fukazawa, Takeshi Ito, Yuji Kamiya, Shinjiro Yamaguchi, Yohsuke Takahashi

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Gibberellins (GAs) are important phytohormones for plant growth and development. DELLAs are members of the plant-specific GRAS protein family and act as repressors of GA signaling. DELLAs are rapidly degraded in the presence of GAs. GA-GID1-DELLA complexes are recognized and ubiquitinated by the SCFSLY complex. The sleepy1 (sly1) F-box mutant exhibits dwarfism and low-germination phenotypes due to high accumulation of DELLAs. Overexpression of GID1 in the sly1 mutant partially rescues these phenotypes without degradation of DELLAs suggesting that proteolysis independent regulation of DELLAs exists in GA signaling. But the molecular mechanisms of non-proteolytic regulation of DELLA are largely unknown. Recently we identified a DELLA binding transcription factor, GAI-ASSOCIATED FACTOR1 (GAF1). GAF1 also interacts with co-repressor TOPLESS RELATED (TPR) in nuclei. DELLAs and TPR act as coactivator and corepressor of GAF1, respectively. GAs converts the GAF1 complex from transcriptional activator to repressor via degradation of DELLAs. The overexpression of DPAM, lacking of DELLAs binding region of GAF1, partially rescue dwarf phenotypes of GA deficient or GA insensitive mutant. In this study, we investigate the relationship between nonproteolytic regulation of DELLAs and GA signaling via DELLA-GAF1 complex using modified yeast two-hybrid system.

    Original languageEnglish
    Article numbere1052923
    JournalPlant Signaling and Behavior
    Volume10
    Issue number10
    DOIs
    Publication statusPublished - 2015 Jan 1

    Keywords

    • DELLA
    • GAF1
    • GID1
    • Gibberellin
    • Proteolysis
    • SLY1
    • Signaling
    • TOPLESS
    • Transcription factor

    ASJC Scopus subject areas

    • Plant Science

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