Binding of CO at the Pro2 side is crucial for the activation of CO-sensing transcriptional activator CooA. 1H NMR spectroscopic studies

Katsuhiko Yamamoto, Haruto Ishikawa, Satoshi Takahashi, Koichiro Ishimori, Isao Morishima, Hiroshi Nakajima, Shigetoshi Aono

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54 Citations (Scopus)

Abstract

CooA is a heme-containing transcriptional activator that anaerobically binds to DNA at CO atmosphere. To obtain information on the conformational transition of CooA induced by CO binding to the heme, we assigned ring current-shifted 1H NMR signals of CooA using two mutants whose axial ligands of the heme were replaced. In the absence of CO, the NMR spectral pattern of H77Y CooA, in which the axial histidine (His77) was replaced with tyrosine, was similar to that of wild-type CooA. In contrast, the spectra of CooAΔN5, in which the NH2 termini including the other axial ligand (Pro2) were deleted, were drastically modulated. We assigned three signals of wild-type CooA at -4.5, -3.6, and -2.8 ppm to δ1-, α-, and δ2-protons of Pro 2, respectively. The Pro2 signals were undetectable in the upfield region of the spectrum of the CO-bound state, which confirms that CO displaces Pro2. Interestingly, the Pro2 signals were observed for CO-bound H77Y CooA, implying that CO binds to the trans position of Pro2 in H77Y CooA. The abolished CO-dependent transcriptional activity of H77Y CooA is therefore the consequence of Pro2 ligation. These observations are consistent with the view that the movement of the NH 2 terminus triggers the conformational transition to the DNA binding form.

Original languageEnglish
Pages (from-to)11473-11476
Number of pages4
JournalJournal of Biological Chemistry
Volume276
Issue number15
DOIs
Publication statusPublished - 2001 Apr 13
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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