Benzophenones from an endophytic fungus, graphiopsis chlorocephala, from Paeonia lactiflora cultivated in the presence of an NAD⁺-dependent HDAC inhibitor

Graphiopsis chlorocephala was separated from the surface-sterilized healthy leaves of Paeonia lactiflora (Paeoniaceae) and cultivated with nicotinamide (an NAD⁺-dependent HDAC inhibitor). The culture conditions significantly enhanced secondary metabolite production in the fungus and led to the isolation of a structurally diverse set of new benzophenones, cephalanones A-F (1-6), and a known 2-(2,6-dihydroxy-4-methylbenzoyl)-6-hydroxybenzoic acid (7). The structures of 1-6 were determined from NMR data, single crystal X-ray diffraction, and chemical transformations.