Basic fibroblast growth factor induces luteinizing hormone receptor expression in the presence of insulin-like growth factor-I in ovarian granulosa cells

Makoto Kanzaki, Masa aki Hattori, Ryuya Horiuchi, Itaru Kojima

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Effect of basic fibroblast growth factor (bFGF) on the expression of receptors for luteinizing hormone (LH), a marker of differentiation, was studied using estrogen-primed rat ovarian granulosa cells in primary culture. bFGF had no effect by itself but dose-dependently induced expression of functional LH receptors in the presence of insulin-like growth factor-I (IGF-I). The effect of a combination of bFGF and IGF-I was delayed in onset and the magnitude of the response was smaller when compared to the action of follicle-stimulating hormone (FSH). Scatchard analysis revealed that disspciation constant (Kd) and number of LH receptors induced by bFGF and IGF-I were 0.47 nM and 6.48 fmol/106 cells, respectively. Unlike FSH, bFGF plus IGF-I did not cause an immediate increase in cAMP release, however, considerable amount of cAMP release was observed in cells incubated for 72 h with bFGF plus IGF-I. Indomethacin, an inhibitor of cyclooxygenase, attenuated both LH receptor expression and cAMP release induced by bFGF plus IGF-I but had little effect on the action of FSH. Finally, a combination of bFGF and IGF-I increased production of prostaglandin E2 in granulosa cells. These results indicate that bFGF is capable of inducing LH receptor in the presence of IGF-I by a mechanism involving production of prostaglandin E2.

Original languageEnglish
Pages (from-to)95-99
Number of pages5
JournalMolecular and Cellular Endocrinology
Volume101
Issue number1-2
DOIs
Publication statusPublished - 1994 May
Externally publishedYes

Keywords

  • Fibroblast growth factor
  • Granulosa cell differentiation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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