Base-pair mapping by chemical force microscopy on nucleobase self-assembled monolayers

K. Ijiro; H. Sunami; K. Arai; J. Matsumoto; O. Karthaus; S. Kraemer; S. Mittler; N. Nishi; B. Juskowiak; S. Takenaka; W. Knoll; M. Shimomura

doi:10.1016/S0927-7757(01)00983-9

Base-pair mapping by chemical force microscopy on nucleobase self-assembled monolayers

K. Ijiro, H. Sunami, K. Arai, J. Matsumoto, O. Karthaus, S. Kraemer, S. Mittler, N. Nishi, B. Juskowiak, S. Takenaka, W. Knoll, M. Shimomura

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Self-assembled monolayers (SAMs) of double-chain disulfide derivatives of nucleobases (adenine and thymine) were formed on Au substrates in order to measure complementary hydrogen bonding by chemical force microscopy at the interfaces. Surface plasmon resonance measurements indicated that the formation of the nucleobase SAMs on Au surface was completed within 80 min. To measure adhesion force by atomic force microscopy (AFM), Au-coated AFM tips were modified with the nucleobase SAMs, too. SAM-modified Au substrates micro-patterned on quartz substrates were prepared for adhesion force mapping. The adhesion force between the complementary nucleobases is larger than that of the non-complementary combination. Electrochemical detection using a redox-intercalator was demonstrated for hybridization of single-stranded polynucleic acid with the nucleobase SAMs modified on Au electrodes.

Original language	English
Pages (from-to)	677-682
Number of pages	6
Journal	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volume	198-200
DOIs	https://doi.org/10.1016/S0927-7757(01)00983-9
Publication status	Published - 2002 Feb 18

Keywords

Base pair
Chemical force mapping
Hydrogen bond
Nucleic acid base
Self-assembled monolayer

ASJC Scopus subject areas

Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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Ijiro, K., Sunami, H., Arai, K., Matsumoto, J., Karthaus, O., Kraemer, S., Mittler, S., Nishi, N., Juskowiak, B., Takenaka, S., Knoll, W., & Shimomura, M. (2002). Base-pair mapping by chemical force microscopy on nucleobase self-assembled monolayers. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 198-200, 677-682. https://doi.org/10.1016/S0927-7757(01)00983-9

Base-pair mapping by chemical force microscopy on nucleobase self-assembled monolayers. / Ijiro, K.; Sunami, H.; Arai, K.; Matsumoto, J.; Karthaus, O.; Kraemer, S.; Mittler, S.; Nishi, N.; Juskowiak, B.; Takenaka, S.; Knoll, W.; Shimomura, M.

In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 198-200, 18.02.2002, p. 677-682.

Research output: Contribution to journal › Article › peer-review

Ijiro, K, Sunami, H, Arai, K, Matsumoto, J, Karthaus, O, Kraemer, S, Mittler, S, Nishi, N, Juskowiak, B, Takenaka, S, Knoll, W & Shimomura, M 2002, 'Base-pair mapping by chemical force microscopy on nucleobase self-assembled monolayers', Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 198-200, pp. 677-682. https://doi.org/10.1016/S0927-7757(01)00983-9

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abstract = "Self-assembled monolayers (SAMs) of double-chain disulfide derivatives of nucleobases (adenine and thymine) were formed on Au substrates in order to measure complementary hydrogen bonding by chemical force microscopy at the interfaces. Surface plasmon resonance measurements indicated that the formation of the nucleobase SAMs on Au surface was completed within 80 min. To measure adhesion force by atomic force microscopy (AFM), Au-coated AFM tips were modified with the nucleobase SAMs, too. SAM-modified Au substrates micro-patterned on quartz substrates were prepared for adhesion force mapping. The adhesion force between the complementary nucleobases is larger than that of the non-complementary combination. Electrochemical detection using a redox-intercalator was demonstrated for hybridization of single-stranded polynucleic acid with the nucleobase SAMs modified on Au electrodes.",

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AU - Karthaus, O.

AU - Kraemer, S.

AU - Mittler, S.

AU - Nishi, N.

AU - Juskowiak, B.

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AU - Knoll, W.

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AB - Self-assembled monolayers (SAMs) of double-chain disulfide derivatives of nucleobases (adenine and thymine) were formed on Au substrates in order to measure complementary hydrogen bonding by chemical force microscopy at the interfaces. Surface plasmon resonance measurements indicated that the formation of the nucleobase SAMs on Au surface was completed within 80 min. To measure adhesion force by atomic force microscopy (AFM), Au-coated AFM tips were modified with the nucleobase SAMs, too. SAM-modified Au substrates micro-patterned on quartz substrates were prepared for adhesion force mapping. The adhesion force between the complementary nucleobases is larger than that of the non-complementary combination. Electrochemical detection using a redox-intercalator was demonstrated for hybridization of single-stranded polynucleic acid with the nucleobase SAMs modified on Au electrodes.

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