Bactericidal effect of hydroxyl radical generated by photolysis of hydrogen peroxide

Hiroyo Ikai; Keisuke Nakamura; Midori Shirato; Taro Kanno; Atsuo Iwasawa; Yoshimi Niwano; Keiichi Sasaki; Masahiro Kohno

doi:10.1007/978-4-431-54070-0_14

Bactericidal effect of hydroxyl radical generated by photolysis of hydrogen peroxide

Hiroyo Ikai, Keisuke Nakamura, Midori Shirato, Taro Kanno, Atsuo Iwasawa, Yoshimi Niwano, Keiichi Sasaki, Masahiro Kohno

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Abstract

The relationship between the yield of hydroxyl radical generated by photolysis of H₂O₂ and its bactericidal effect was evaluated. To generate hydroxyl radical, H₂O₂ was irradiated with laser light at a wavelength of 405 nm. ESR spin trapping analysis showed that the yield of hydroxyl radical increased with either the laser irradiation time or the concentration of H₂O₂. Streptococcus mutans and Porphyromonas gingivalis were used in the bactericidal assay. The assay showed that the bactericidal effect was related to the laser irradiation time and the concentration of H₂O₂. Laser irradiation of the planktonic and the biofilm model of S. mutans in 1,000 mM H₂O₂ and P. gingivalis in 500 mM showed a >5-log reduction of viable counts within 3 min and 30 s, respectively. These results suggest that the bactericidal activity against oral pathogenic bacteria consisting of facultative and obligate anaerobes is dependent on the yield of hydroxyl radical.

Original language	English
Title of host publication	Interface Oral Health Science 2011
Publisher	Springer Japan
Pages	86-88
Number of pages	3
ISBN (Electronic)	9784431540700
ISBN (Print)	9784431540694
DOIs	https://doi.org/10.1007/978-4-431-54070-0_14
Publication status	Published - 2012 Jan 1

Keywords

Bactericidal effect
Biofilm
Hydrogen peroxide
Hydroxyl radical
Photolysis

ASJC Scopus subject areas

Dentistry(all)

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10.1007/978-4-431-54070-0_14

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title = "Bactericidal effect of hydroxyl radical generated by photolysis of hydrogen peroxide",

abstract = "The relationship between the yield of hydroxyl radical generated by photolysis of H2O2 and its bactericidal effect was evaluated. To generate hydroxyl radical, H2O2 was irradiated with laser light at a wavelength of 405 nm. ESR spin trapping analysis showed that the yield of hydroxyl radical increased with either the laser irradiation time or the concentration of H2O2. Streptococcus mutans and Porphyromonas gingivalis were used in the bactericidal assay. The assay showed that the bactericidal effect was related to the laser irradiation time and the concentration of H2O2. Laser irradiation of the planktonic and the biofilm model of S. mutans in 1,000 mM H2O2 and P. gingivalis in 500 mM showed a >5-log reduction of viable counts within 3 min and 30 s, respectively. These results suggest that the bactericidal activity against oral pathogenic bacteria consisting of facultative and obligate anaerobes is dependent on the yield of hydroxyl radical. ",

keywords = "Bactericidal effect, Biofilm, Hydrogen peroxide, Hydroxyl radical, Photolysis",

author = "Hiroyo Ikai and Keisuke Nakamura and Midori Shirato and Taro Kanno and Atsuo Iwasawa and Yoshimi Niwano and Keiichi Sasaki and Masahiro Kohno",

year = "2012",

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doi = "10.1007/978-4-431-54070-0_14",

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T1 - Bactericidal effect of hydroxyl radical generated by photolysis of hydrogen peroxide

AU - Ikai, Hiroyo

AU - Nakamura, Keisuke

AU - Shirato, Midori

AU - Kanno, Taro

AU - Iwasawa, Atsuo

AU - Niwano, Yoshimi

AU - Sasaki, Keiichi

AU - Kohno, Masahiro

PY - 2012/1/1

Y1 - 2012/1/1

N2 - The relationship between the yield of hydroxyl radical generated by photolysis of H2O2 and its bactericidal effect was evaluated. To generate hydroxyl radical, H2O2 was irradiated with laser light at a wavelength of 405 nm. ESR spin trapping analysis showed that the yield of hydroxyl radical increased with either the laser irradiation time or the concentration of H2O2. Streptococcus mutans and Porphyromonas gingivalis were used in the bactericidal assay. The assay showed that the bactericidal effect was related to the laser irradiation time and the concentration of H2O2. Laser irradiation of the planktonic and the biofilm model of S. mutans in 1,000 mM H2O2 and P. gingivalis in 500 mM showed a >5-log reduction of viable counts within 3 min and 30 s, respectively. These results suggest that the bactericidal activity against oral pathogenic bacteria consisting of facultative and obligate anaerobes is dependent on the yield of hydroxyl radical.

AB - The relationship between the yield of hydroxyl radical generated by photolysis of H2O2 and its bactericidal effect was evaluated. To generate hydroxyl radical, H2O2 was irradiated with laser light at a wavelength of 405 nm. ESR spin trapping analysis showed that the yield of hydroxyl radical increased with either the laser irradiation time or the concentration of H2O2. Streptococcus mutans and Porphyromonas gingivalis were used in the bactericidal assay. The assay showed that the bactericidal effect was related to the laser irradiation time and the concentration of H2O2. Laser irradiation of the planktonic and the biofilm model of S. mutans in 1,000 mM H2O2 and P. gingivalis in 500 mM showed a >5-log reduction of viable counts within 3 min and 30 s, respectively. These results suggest that the bactericidal activity against oral pathogenic bacteria consisting of facultative and obligate anaerobes is dependent on the yield of hydroxyl radical.

KW - Bactericidal effect

KW - Biofilm

KW - Hydrogen peroxide

KW - Hydroxyl radical

KW - Photolysis

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DO - 10.1007/978-4-431-54070-0_14

M3 - Chapter

AN - SCOPUS:84956968581

SN - 9784431540694

SP - 86

EP - 88

BT - Interface Oral Health Science 2011

PB - Springer Japan

ER -

Bactericidal effect of hydroxyl radical generated by photolysis of hydrogen peroxide

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Keywords

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