Bacterial community dynamics during the preferential degradation of aromatic hydrocarbons by a microbial consortium

Hernando Pactao Bacosa, Koichi Suto, Chihiro Inoue

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)

Abstract

The aims of this study were to identify the bacteria responsible for the rapid degradation of aromatic hydrocarbons, and to determine the roles of bacterial species in the microbial consortium. The population changes of the four major bacterial groups, namely Burkholderia spp., Achromobacter spp./. Alcaligenes spp., Cupriavidus spp., and Rhodanobacter spp. in the Y consortium that preferentially degraded petroleum oil-derived aromatic hydrocarbons were assessed using quantitative real-time polymerase chain reaction (q-PCR). P-xylene was used as a representative aromatic compound, decane as a representative aliphatic compound, p-xylene-decane as a representative hydrocarbon mixture, and kerosene as a representative petroleum-based oil. The results showed that the remarkable increase of Burkholderia spp. coincided with the rapid degradation of aromatic hydrocarbons in kerosene, and p-xylene in single and dual-substrate cultures. Cupriavidus spp. significantly increased when decane was rapidly depleted. Achromobacter spp.,/. Alcaligenes spp., and Rhodanobacter spp. were initially inhibited by the presence of aromatic hydrocarbons and seemed not to be active in hydrocarbon degradation, but utilized the metabolic products. This work provided an alternative way to explain the contribution of different microbial species in the degradation of hydrocarbon fractions. The behavior of the consortium is of interest in the context of the bioremediation of the more toxic aromatic hydrocarbons in environments contaminated by petroleum products.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalInternational Biodeterioration and Biodegradation
Volume74
DOIs
Publication statusPublished - 2012 Oct

Keywords

  • Aromatic hydrocarbon
  • Biodegradation
  • Burkholderia spp.
  • Kerosene
  • Microbial consortium
  • Real-time PCR

ASJC Scopus subject areas

  • Microbiology
  • Biomaterials
  • Waste Management and Disposal

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