Bach1 modulates heme oxygenase-1 expression in the neonatal mouse lung

Sacha Kassovska-Bratinova, Guang Yang, Kazuhiko Igarashi, Phyllis A. Dennery

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Heme oxygenase-1 (HO-1), the rate-limiting enzyme of heme degradation and antioxidant defense protein, is induced in the lungs of animals exposed to hyperoxia. However, high levels of HO-1 expression may be deleterious, thus necessitating tight regulation. Previous reports show maturational differences in rat HO-1 regulation in hyperoxia, as newborns do not up-regulate HO-1mRNA compared with adults. To better understand the differential response of lung HO-1 to hyperoxia, we exposed newborn and adult mice to >95% oxygen. The newborn lungs had reduced HO-1 mRNA induction compared with adults and newborn transgenic mice over-expressing luciferase driven by the 15 kb HO-1 promoter (HO-1/Luc Tg) had less increased light emission in hyperoxia compared with adults. Compared with adults, levels of the represser of HO-1 transcription, Bach1, were higher in the neonatal lung as was nuclear protein-DNA binding to the antioxidant response element (ARE) from HO-1. Furthermore, at baseline and in hyperoxia, chromatin immunoprecipitation (ChIP) revealed increased Bach1 binding to the HO-1 distal enhancers (DEs) in the neonates compared with adults. These data suggest that elevated levels of Bach1 may help to limit HO-1 induction in the newborn at baseline and in response to oxidative stress.

Original languageEnglish
Pages (from-to)145-149
Number of pages5
JournalPediatric Research
Volume65
Issue number2
DOIs
Publication statusPublished - 2009 Feb

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

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