Babesia microti: Molecular and antigenic characterizations of a novel 94-kDa protein (BmP94)

Hideo Ooka, Mohamad Alaa Terkawi, Youn Kyoung Goo, Yuzi Luo, Yan Li, Junya Yamagishi, Yoshifumi Nishikawa, Ikuo Igarashi, Xuenan Xuan

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

A novel gene, BmP94, encoding 94-kDa protein of Babesia microti was identified by immunoscreening of the cDNA expression library. The full-length of BmP94 was expressed in Escherichia coli (rBmP94), which resulted in insoluble form with low yield, and the truncated hydrophilic C-terminus region of the gene was expressed as a soluble protein (rBmP94/CT) with improved productivity. Antiserum raised against rBmP94/CT recognized the 94-kDa native protein in the parasite extract by Western blot analysis. Next, an ELISA using rBmP94/CT was evaluated for diagnostic use, and it demonstrated high sensitivity and specificity when tested with the sera from mice experimentally infected with B. microti and closely related parasites. Moreover, the immunoprotective property of rBmP94/CT as a subunit vaccine was evaluated in BALB/c mice against a B. microti challenge, but no significant protection was observed. Our data suggest that the immunodominant antigen BmP94 could be a promising candidate for diagnostic use for human babesiosis.

Original languageEnglish
Pages (from-to)287-293
Number of pages7
JournalExperimental Parasitology
Volume127
Issue number1
DOIs
Publication statusPublished - 2011 Jan
Externally publishedYes

Keywords

  • Babesia microti
  • BmP94
  • ELISA
  • Vaccination

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

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