B-Cell Mitogen Produced by Slime-Forming, Encapsulated Lactococcus lactis ssp. cremoris Isolated from Ropy Sour Milk, Viili

Haruki Kitazawa, Takahiro Yamaguchi, Makoto Miura, Tadao Saito, Takatoshi Itoh

    Research output: Contribution to journalArticlepeer-review

    43 Citations (Scopus)

    Abstract

    A substance, active as a B-cell mitogen, was isolated from the slime products produced by Lactococcus lactis ssp. cremoris KVS20. The mitogenic substance was prepared by anion-exchange chromatography and gel filtration chromatography and then purified by proteinase digestion and HPLC. Chemical analysis determined that the mitogenic substance was a phosphopolysaccharide and consisted of rhamnose, glucose, galactose, and phosphorus. The activity of the mitogenic substance was higher than that of the slime products. The optimal concentration for the activity was approximately 120 μg/ml. The mitogenic substance also had substantial mitogenic activity to spleen cells from C3H/HeJ mice, which are resistant to lipopolysaccharide. The findings indicated that a B-cell mitogen different from lipopolysaccharide is produced from L. lactis ssp. cremoris KVS20.

    Original languageEnglish
    Pages (from-to)1514-1519
    Number of pages6
    JournalJournal of Dairy Science
    Volume76
    Issue number6
    DOIs
    Publication statusPublished - 1993

    Keywords

    • B-cell mitogen
    • CSP
    • LPS
    • Lactococcus lactis ssp. cremoris
    • SI
    • cellular slime product produced by Lactococcus lactis ssp. cremoris KVS20
    • lipopolysaccharide
    • phospho-polysaccharide
    • slime product
    • stimulation index

    ASJC Scopus subject areas

    • Food Science
    • Animal Science and Zoology
    • Genetics

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