AxyR, an AraC family transcriptional activator of the xylanase 3 gene, requires xylo-oligosaccharides as a cofactor for DNA binding in Paenibacillus sp. strain W-61

Mutsumi Fukuda; Shigeki Yoshida; Hiroya Itoh; Yoji Kato; Seiji Watanabe; Yoshifumi Itoh; Yoshiyuki Kamio; Jun Kaneko

doi:10.1271/bbb.120165

AxyR, an AraC family transcriptional activator of the xylanase 3 gene, requires xylo-oligosaccharides as a cofactor for DNA binding in Paenibacillus sp. strain W-61

Mutsumi Fukuda, Shigeki Yoshida, Hiroya Itoh, Yoji Kato, Seiji Watanabe, Yoshifumi Itoh, Yoshiyuki Kamio, Jun Kaneko

AGR - Bioscience and Biotechnology for Future Bioindustries

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Abstract

The xylanolytic bacterium Paenibacillus sp. strain W-61 encodes three extracellular xylanase genes, xyn1, xyn3, and xyn5. In this study, we identified a transcriptional activator required for transcription of the xyn3 gene in strain W-61. The activator, AxyR, contained the highly homologous AraC-type DNA binding domain and required xylobiose, xylotriose, or xylotetraose as cofactor for binding to the xyn3 promoter region.

Original language	English
Pages (from-to)	1051-1054
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	76
Issue number	5
DOIs	https://doi.org/10.1271/bbb.120165
Publication status	Published - 2012

Keywords

Co-factor
DNA-binding protein
Paenibacillus
Xylanase
Xylooligosaccharides

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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AxyR, an AraC family transcriptional activator of the xylanase 3 gene, requires xylo-oligosaccharides as a cofactor for DNA binding in Paenibacillus sp. strain W-61. / Fukuda, Mutsumi; Yoshida, Shigeki; Itoh, Hiroya; Kato, Yoji; Watanabe, Seiji; Itoh, Yoshifumi; Kamio, Yoshiyuki; Kaneko, Jun.

In: Bioscience, Biotechnology and Biochemistry, Vol. 76, No. 5, 2012, p. 1051-1054.

Research output: Contribution to journal › Article › peer-review

Fukuda, Mutsumi ; Yoshida, Shigeki ; Itoh, Hiroya ; Kato, Yoji ; Watanabe, Seiji ; Itoh, Yoshifumi ; Kamio, Yoshiyuki ; Kaneko, Jun. / AxyR, an AraC family transcriptional activator of the xylanase 3 gene, requires xylo-oligosaccharides as a cofactor for DNA binding in Paenibacillus sp. strain W-61. In: Bioscience, Biotechnology and Biochemistry. 2012 ; Vol. 76, No. 5. pp. 1051-1054.

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abstract = "The xylanolytic bacterium Paenibacillus sp. strain W-61 encodes three extracellular xylanase genes, xyn1, xyn3, and xyn5. In this study, we identified a transcriptional activator required for transcription of the xyn3 gene in strain W-61. The activator, AxyR, contained the highly homologous AraC-type DNA binding domain and required xylobiose, xylotriose, or xylotetraose as cofactor for binding to the xyn3 promoter region.",

keywords = "Co-factor, DNA-binding protein, Paenibacillus, Xylanase, Xylooligosaccharides",

author = "Mutsumi Fukuda and Shigeki Yoshida and Hiroya Itoh and Yoji Kato and Seiji Watanabe and Yoshifumi Itoh and Yoshiyuki Kamio and Jun Kaneko",

note = "Funding Information: We are grateful to Dr. Katsuya Gomi and Dr. Hiroshi Yoneyama (Tohoku University, Sendai, Japan) for helpful advice and comments on the manuscript. This study was supported in part by the Hayashi Memorial Foundation for Female Natural Scientists of Japan and the program Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists (to M.F.). Copyright: Copyright 2013 Elsevier B.V., All rights reserved.",

year = "2012",

doi = "10.1271/bbb.120165",

language = "English",

volume = "76",

pages = "1051--1054",

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AU - Fukuda, Mutsumi

AU - Yoshida, Shigeki

AU - Itoh, Hiroya

AU - Kato, Yoji

AU - Watanabe, Seiji

AU - Itoh, Yoshifumi

AU - Kamio, Yoshiyuki

AU - Kaneko, Jun

N1 - Funding Information: We are grateful to Dr. Katsuya Gomi and Dr. Hiroshi Yoneyama (Tohoku University, Sendai, Japan) for helpful advice and comments on the manuscript. This study was supported in part by the Hayashi Memorial Foundation for Female Natural Scientists of Japan and the program Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists (to M.F.). Copyright: Copyright 2013 Elsevier B.V., All rights reserved.

PY - 2012

Y1 - 2012

N2 - The xylanolytic bacterium Paenibacillus sp. strain W-61 encodes three extracellular xylanase genes, xyn1, xyn3, and xyn5. In this study, we identified a transcriptional activator required for transcription of the xyn3 gene in strain W-61. The activator, AxyR, contained the highly homologous AraC-type DNA binding domain and required xylobiose, xylotriose, or xylotetraose as cofactor for binding to the xyn3 promoter region.

AB - The xylanolytic bacterium Paenibacillus sp. strain W-61 encodes three extracellular xylanase genes, xyn1, xyn3, and xyn5. In this study, we identified a transcriptional activator required for transcription of the xyn3 gene in strain W-61. The activator, AxyR, contained the highly homologous AraC-type DNA binding domain and required xylobiose, xylotriose, or xylotetraose as cofactor for binding to the xyn3 promoter region.

KW - Co-factor

KW - DNA-binding protein

KW - Paenibacillus

KW - Xylanase

KW - Xylooligosaccharides

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JO - Bioscience, Biotechnology and Biochemistry

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AxyR, an AraC family transcriptional activator of the xylanase 3 gene, requires xylo-oligosaccharides as a cofactor for DNA binding in Paenibacillus sp. strain W-61

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