AxyR, an AraC family transcriptional activator of the xylanase 3 gene, requires xylo-oligosaccharides as a cofactor for DNA binding in Paenibacillus sp. strain W-61

Mutsumi Fukuda, Shigeki Yoshida, Hiroya Itoh, Yoji Kato, Seiji Watanabe, Yoshifumi Itoh, Yoshiyuki Kamio, Jun Kaneko

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)

    Abstract

    The xylanolytic bacterium Paenibacillus sp. strain W-61 encodes three extracellular xylanase genes, xyn1, xyn3, and xyn5. In this study, we identified a transcriptional activator required for transcription of the xyn3 gene in strain W-61. The activator, AxyR, contained the highly homologous AraC-type DNA binding domain and required xylobiose, xylotriose, or xylotetraose as cofactor for binding to the xyn3 promoter region.

    Original languageEnglish
    Pages (from-to)1051-1054
    Number of pages4
    JournalBioscience, Biotechnology and Biochemistry
    Volume76
    Issue number5
    DOIs
    Publication statusPublished - 2012

    Keywords

    • Co-factor
    • DNA-binding protein
    • Paenibacillus
    • Xylanase
    • Xylooligosaccharides

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

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