AxyR, an AraC family transcriptional activator of the xylanase 3 gene, requires xylo-oligosaccharides as a cofactor for DNA binding in Paenibacillus sp. strain W-61

Mutsumi Fukuda, Shigeki Yoshida, Hiroya Itoh, Yoji Kato, Seiji Watanabe, Yoshifumi Itoh, Yoshiyuki Kamio, Jun Kaneko

Research output: Contribution to journalArticlepeer-review

Abstract

The xylanolytic bacterium Paenibacillus sp. strain W-61 encodes three extracellular xylanase genes, xyn1, xyn3, and xyn5. In this study, we identified a transcriptional activator required for transcription of the xyn3 gene in strain W-61. The activator, AxyR, contained the highly homologous AraC-type DNA binding domain and required xylobiose, xylotriose, or xylotetraose as cofactor for binding to the xyn3 promoter region.

Original languageEnglish
Pages (from-to)1051-1054
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume76
Issue number5
DOIs
Publication statusPublished - 2012 May 29

Keywords

  • Co-factor
  • DNA-binding protein
  • Paenibacillus
  • Xylanase
  • Xylooligosaccharides

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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