AxyR, an AraC family transcriptional activator of the xylanase 3 gene, requires xylo-oligosaccharides as a cofactor for DNA binding in Paenibacillus sp. strain W-61

Mutsumi Fukuda; Shigeki Yoshida; Hiroya Itoh; Yoji Kato; Seiji Watanabe; Yoshifumi Itoh; Yoshiyuki Kamio; Jun Kaneko

doi:10.1271/bbb.120165

AxyR, an AraC family transcriptional activator of the xylanase 3 gene, requires xylo-oligosaccharides as a cofactor for DNA binding in Paenibacillus sp. strain W-61

Mutsumi Fukuda, Shigeki Yoshida, Hiroya Itoh, Yoji Kato, Seiji Watanabe, Yoshifumi Itoh, Yoshiyuki Kamio, Jun Kaneko

AGR - Bioscience and Biotechnology for Future Bioindustries

Research output: Contribution to journal › Article › peer-review

Abstract

The xylanolytic bacterium Paenibacillus sp. strain W-61 encodes three extracellular xylanase genes, xyn1, xyn3, and xyn5. In this study, we identified a transcriptional activator required for transcription of the xyn3 gene in strain W-61. The activator, AxyR, contained the highly homologous AraC-type DNA binding domain and required xylobiose, xylotriose, or xylotetraose as cofactor for binding to the xyn3 promoter region.

Original language	English
Pages (from-to)	1051-1054
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	76
Issue number	5
DOIs	https://doi.org/10.1271/bbb.120165
Publication status	Published - 2012 May 29

Keywords

Co-factor
DNA-binding protein
Paenibacillus
Xylanase
Xylooligosaccharides

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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AxyR, an AraC family transcriptional activator of the xylanase 3 gene, requires xylo-oligosaccharides as a cofactor for DNA binding in Paenibacillus sp. strain W-61. / Fukuda, Mutsumi; Yoshida, Shigeki; Itoh, Hiroya; Kato, Yoji; Watanabe, Seiji; Itoh, Yoshifumi; Kamio, Yoshiyuki; Kaneko, Jun.

In: Bioscience, Biotechnology and Biochemistry, Vol. 76, No. 5, 29.05.2012, p. 1051-1054.

Research output: Contribution to journal › Article › peer-review

Fukuda, Mutsumi ; Yoshida, Shigeki ; Itoh, Hiroya ; Kato, Yoji ; Watanabe, Seiji ; Itoh, Yoshifumi ; Kamio, Yoshiyuki ; Kaneko, Jun. / AxyR, an AraC family transcriptional activator of the xylanase 3 gene, requires xylo-oligosaccharides as a cofactor for DNA binding in Paenibacillus sp. strain W-61. In: Bioscience, Biotechnology and Biochemistry. 2012 ; Vol. 76, No. 5. pp. 1051-1054.

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abstract = "The xylanolytic bacterium Paenibacillus sp. strain W-61 encodes three extracellular xylanase genes, xyn1, xyn3, and xyn5. In this study, we identified a transcriptional activator required for transcription of the xyn3 gene in strain W-61. The activator, AxyR, contained the highly homologous AraC-type DNA binding domain and required xylobiose, xylotriose, or xylotetraose as cofactor for binding to the xyn3 promoter region.",

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AU - Fukuda, Mutsumi

AU - Yoshida, Shigeki

AU - Itoh, Hiroya

AU - Kato, Yoji

AU - Watanabe, Seiji

AU - Itoh, Yoshifumi

AU - Kamio, Yoshiyuki

AU - Kaneko, Jun

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AB - The xylanolytic bacterium Paenibacillus sp. strain W-61 encodes three extracellular xylanase genes, xyn1, xyn3, and xyn5. In this study, we identified a transcriptional activator required for transcription of the xyn3 gene in strain W-61. The activator, AxyR, contained the highly homologous AraC-type DNA binding domain and required xylobiose, xylotriose, or xylotetraose as cofactor for binding to the xyn3 promoter region.

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