Axonal supercharging technique using reverse end-to-side neurorrhaphy in peripheral nerve repair: An experimental study in the rat model

Toshihiro Fujiwara, Ken Matsuda, Tateki Kubo, Koichi Tomita, Ryo Hattori, Takeshi Masuoka, Kenji Yano, Ko Hosokawa

    Research output: Contribution to journalArticlepeer-review

    36 Citations (Scopus)

    Abstract

    Object. In an attempt to improve peripheral nerve repair, the influence of the addition of reverse end-to-side neurorrhaphy for an injured peripheral nerve was investigated in the rat sciatic nerve transection model. Methods. Twelve Sprague-Dawley rats were divided into two groups (six rats in each group). In Group I, the right sciatic nerve was cut at a point distal to the gluteal notch and repaired using end-to-end neurorrhaphy with four 10-0 nylon epineurial sutures. In Group II, after performing the same procedure as in Group I, the left sciatic nerve was cut distally and passed through a subcutaneous tunnel to the right side. The proximal stump of the left sciatic nerve was coapted to the epineurial window of the right sciatic nerve distal to the injured point in an end-to-side fashion using 10-0 nylon epineurial sutures. The effects were evaluated using analgesimeter recordings for the hind paw, electrophysiological tests, measurement of the muscle contraction force, a double-labeling technique, weight measurement and histological examination of the gastrocnemius muscle, histological examination of the bilateral sciatic nerves, and immunofluorescent staining. Results. Results from the many tests used to evaluate the reverse end-to-side neurorrhaphy technique indicated that functional recovery of the denervated target organs was promoted by axonal augmentation. Conclusions. The reverse end-to-side neurorrhaphy technique could be useful in peripheral nerve repair.

    Original languageEnglish
    Pages (from-to)821-829
    Number of pages9
    JournalJournal of Neurosurgery
    Volume107
    Issue number4
    DOIs
    Publication statusPublished - 2007 Oct

    Keywords

    • Animal model
    • End-to-side nerve repair
    • Nerve regeneration
    • Neurorrhaphy
    • Peripheral nerve

    ASJC Scopus subject areas

    • Clinical Neurology
    • Neuroscience(all)

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