Avidin/PSS membrane microcapsules with biotin-binding activity

Yoshihiro Endo, Katsuhiko Sato, Kentaro Sugimoto, Jun ichi Anzai

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Polyelectrolyte microcapsules with avidin-poly(styrene sulfonate) (PSS) membrane were prepared by a layer-by-layer deposition technique. The uptake and release of biotin-labeled fluorescein (b-FITC) as well as immobilization of biotin-labeled glucose oxidase (b-GOx) to the microcapsule were studied. The polyelectrolyte microcapsules were prepared by coating the surface of calcium carbonate (CaCO3) microparticles with an avidin/PSS multilayer membrane, followed by dissolution of CaCO3 core in an ethylenediaminetetraacetic acid solution. Inner and outer poly(allylamine)/PSS films were required to isolate the microcapsules, whereas microcapsules could not be formed without the support. The uptake of b-FITC into the microcapsule was highly enhanced through a strong binding of b-FITC to avidin as compared with the uptake of biotin-free FITC. Release of b-FITC from the microcapsule was accelerated upon addition of biotin due to a competitive binding of the added biotin to the binding site of avidin. Similarly, the surface of microcapsule was modified with b-GOx with retaining its catalytic activity.

Original languageEnglish
Pages (from-to)519-524
Number of pages6
JournalJournal of Colloid And Interface Science
Volume360
Issue number2
DOIs
Publication statusPublished - 2011 Aug 15

Keywords

  • Avidin
  • Biotin
  • Glucose oxidase
  • Layer-by-layer film
  • Polyelectrolyte microcapsule
  • Uptake and release

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry

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