TY - JOUR
T1 - Avidin-biotin complexation for enzyme sensor applications
AU - Anzai, Jun ichi
AU - Hoshi, Tomonori
AU - Osa, Tetsuo
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1994/5
Y1 - 1994/5
N2 - The use of an avidin-biotin system in the preparation of enzyme sensors is described. The biotin-labelled enzymes can be immobilized on the avidin-modified electrode surface through avidin-biotin complexation. The various techniques for the surface derivatization with biotin and avidin and for the coupling with enzymes are cited. The possibility of constructing a protein architecture on the electrode is also discussed: this is based on the non-covalent interaction of avidin and biotin.
AB - The use of an avidin-biotin system in the preparation of enzyme sensors is described. The biotin-labelled enzymes can be immobilized on the avidin-modified electrode surface through avidin-biotin complexation. The various techniques for the surface derivatization with biotin and avidin and for the coupling with enzymes are cited. The possibility of constructing a protein architecture on the electrode is also discussed: this is based on the non-covalent interaction of avidin and biotin.
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U2 - 10.1016/0165-9936(94)85040-2
DO - 10.1016/0165-9936(94)85040-2
M3 - Article
AN - SCOPUS:0028221732
VL - 13
SP - 205
EP - 210
JO - TrAC - Trends in Analytical Chemistry
JF - TrAC - Trends in Analytical Chemistry
SN - 0165-9936
IS - 5
ER -