TY - JOUR
T1 - Autotaxin–lysophosphatidic acid pathway in intraocular pressure regulation and glaucoma subtypes
AU - Honjo, Megumi
AU - Igarashi, Nozomi
AU - Kurano, Makoto
AU - Yatomi, Yutaka
AU - Igarashi, Koji
AU - Kano, Kuniyuki
AU - Aoki, Junken
AU - Weinreb, Robert N.
AU - Aihara, Makoto
N1 - Funding Information:
The authors thank Ryo Asaoka, Hiroshi Murata, Takashi Fujishiro, and Rei Sakata (The University of Tokyo) for providing human aqueous humor samples and the staff of the Department of Ophthalmology, The University of Tokyo, for assistance. The English in this document has been checked by at least two professional editors, both native speakers of English. For a certificate, please see: https://www.bioedit.com/digital-certifi cate/view/8baaab11479ff359441a88befe8f8388a37a5e05. Supported by Japan Society for the Promotion of Science Grant 15K10854 (MH), a Grant-in-Aid for Scientific Research on Innovative Areas 15H05906 (YY), and CREST from Japan Science and Technology Corporation (JST). Disclosure: M. Honjo, None; N. Igarashi, None; M. Kurano, None; Y. Yatomi, None; K. Igarashi, None; K. Kano, None; J. Aoki, None; R.N. Weinreb, None; M. Aihara, None
Publisher Copyright:
© 2018 The Authors.
PY - 2018/2
Y1 - 2018/2
N2 - Purpose: To compare the levels of autotaxin (ATX), lysophosphatidic acid (LPA), and lysophosphatidylcholine (LPC) in the aqueous humor (AH) of healthy control subjects with those of patients with different subtypes of glaucoma, and also to investigate the relationship of the ATX–LPA pathway with IOP and subtype of glaucoma. Methods: This study included 164 eyes of 164 consecutive cases of cataract and glaucoma surgery (37 healthy, 31 normal tension glaucoma, 49 primary open angle glaucoma, 28 secondary open angle glaucoma, and 19 exfoliation glaucoma). Aqueous levels of LPA, LPC, and ATX were quantified using liquid chromatography-tandem mass spectrometry and a two-site immunoenzymetric assay. The association between aqueous levels of ATX/LPA/LPC and IOP elevation in different glaucoma subtypes was investigated. The diagnostic values of indices of the ATX–LPA pathway were compared using receiver operating characteristic curve analysis. Results: Notable increases in ATX/LPA/LPC levels in glaucoma patients were observed. The ATX–LPA pathway was significantly related to IOP elevation and the subtype of glaucoma, especially in SOAG and XFG patients, and the area under the curve was significant for discriminating glaucoma eyes from healthy eyes. Conclusions: Bioactive ATX/LPA/LPC concentrations were present in aqueous humor, and higher ATX and LPA concentrations were significantly correlated with IOP in all study subjects. Furthermore, the ATX–LPA pathway was significantly related to glaucoma subtype. These results reveal the potentially important role of the ATX–LPA pathway for IOP regulation in healthy subjects and glaucoma patients.
AB - Purpose: To compare the levels of autotaxin (ATX), lysophosphatidic acid (LPA), and lysophosphatidylcholine (LPC) in the aqueous humor (AH) of healthy control subjects with those of patients with different subtypes of glaucoma, and also to investigate the relationship of the ATX–LPA pathway with IOP and subtype of glaucoma. Methods: This study included 164 eyes of 164 consecutive cases of cataract and glaucoma surgery (37 healthy, 31 normal tension glaucoma, 49 primary open angle glaucoma, 28 secondary open angle glaucoma, and 19 exfoliation glaucoma). Aqueous levels of LPA, LPC, and ATX were quantified using liquid chromatography-tandem mass spectrometry and a two-site immunoenzymetric assay. The association between aqueous levels of ATX/LPA/LPC and IOP elevation in different glaucoma subtypes was investigated. The diagnostic values of indices of the ATX–LPA pathway were compared using receiver operating characteristic curve analysis. Results: Notable increases in ATX/LPA/LPC levels in glaucoma patients were observed. The ATX–LPA pathway was significantly related to IOP elevation and the subtype of glaucoma, especially in SOAG and XFG patients, and the area under the curve was significant for discriminating glaucoma eyes from healthy eyes. Conclusions: Bioactive ATX/LPA/LPC concentrations were present in aqueous humor, and higher ATX and LPA concentrations were significantly correlated with IOP in all study subjects. Furthermore, the ATX–LPA pathway was significantly related to glaucoma subtype. These results reveal the potentially important role of the ATX–LPA pathway for IOP regulation in healthy subjects and glaucoma patients.
KW - Aqueous humor
KW - Autotaxin
KW - Glaucoma subtypes
KW - Intraocular pressure
KW - Lysophosphatidic acid
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U2 - 10.1167/iovs.17-23218
DO - 10.1167/iovs.17-23218
M3 - Article
C2 - 29392315
AN - SCOPUS:85041551890
VL - 59
SP - 693
EP - 701
JO - Investigative Ophthalmology
JF - Investigative Ophthalmology
SN - 0146-0404
IS - 2
ER -