Autophagy mitigates high-temperature injury in pollen development of Arabidopsis thaliana

Gönül Dündar, Zhenhua Shao, Nahoko Higashitani, Mami Kikuta, Masanori Izumi, Atsushi Higashitani

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Autophagy is one of the cellular processes that break down cellular components during senescence, starvation, and stress. The susceptibility of plant pollen development to high-temperature (HT) stress is well known, but the involvement of autophagy in HT injury is yet to be clarified. Here, we found that following transfer to 30 °C, all autophagy-deficient (atg) mutants (atg2-1, 5-1, 7-2, and 10-1) of Arabidopsis thaliana tested displayed visibly impaired pollen development and anther dehiscence. HT-induced male sterility significantly increased in the atg mutants, but the degree of HT-induced obstacles did not change between the wild type (WT) and mutants from the seedling stage to the bolting stage. Cytological analyses showed that 30 °C promoted autophagy and autolysosome formation in both anther wall cells and microspores in developing anthers of WT, but the atg5-1 mutant did not show completion of tapetum degeneration and microspore maturation. HT upregulated hydrogen peroxide and dehydroascorbate reductase 1 production in both WT and atg5-1 anthers, but the basal levels were already higher in the mutant. HT repressed expression of UNDEAD and its regulator MYB80, which are required for tapetal programmed cell death (PCD) for proper pollen development. Taken together, our results suggest that autophagy functions in tapetum degeneration and pollen development during HT-caused tapetal PCD abortion.

Original languageEnglish
Pages (from-to)190-200
Number of pages11
JournalDevelopmental Biology
Volume456
Issue number2
DOIs
Publication statusPublished - 2019 Dec 15

Keywords

  • ATG
  • High temperature
  • MYB80 signaling
  • Male sterility
  • Oxidative damage
  • PCD
  • Tapetum

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Autophagy mitigates high-temperature injury in pollen development of Arabidopsis thaliana'. Together they form a unique fingerprint.

Cite this