Automatic pseudorotaxane formation targeting on nucleic acids using a pair of reactive oligodeoxynucleotides

Kazumitsu Onizuka, Fumi Nagatsugi, Yoshihiro Ito, Hiroshi Abe

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Here we report a novel method to form a pseudorotaxane architecture using only a pair of reactive oligodeoxyribonucleotides (ODNs), which we designed and synthesized, and then performed the pseudorotaxane formation reaction with both DNA and RNA oligonucleotides. The reaction proceeded smoothly without any extra reagents at 37°C and pH 7.2, leading to the formation of a stable complex on a denaturing polyacrylamide gel. Interestingly, the pseudorotaxane was formed with the cyclized ODN reversibly by the slipping process. This new pseudorotaxane formation represents a promising method for developing new DNA nanotechnologies and antisense oligonucleotides.

Original languageEnglish
Pages (from-to)7201-7204
Number of pages4
JournalJournal of the American Chemical Society
Volume136
Issue number20
DOIs
Publication statusPublished - 2014 May 21

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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