Automated preparation of hypoxic cell marker [18F]FRP-170 by on-column hydrolysis

Yoichi Ishikawa, Ren Iwata, S. Furumoto, Y. Takai

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

An automated synthesis for the preparation of the novel hypoxic cell marker, [18F]FRP-170 3, [18F]1-[2-fluoro-1-(hydroxymethyl) ethoxy]methyl-2- nitroimidazole, was developed using an on-column basic-hydrolysis step. The 18F-labeled protected intermediate 2 was retained on a Sep-Pak Plus C18 cartridge and, in the same cartridge at room temperature, hydrolyzed by NaOH for deacetylation to give [18F]FRP- 170. The elution method from the cartridge was optimized for direct injection of the crude product into an HPLC column. Thus, [18F]FRP-170 was prepared in 20-30% decay-corrected radiochemical yield within 60 min.

Original languageEnglish
Pages (from-to)705-710
Number of pages6
JournalApplied Radiation and Isotopes
Volume62
Issue number5
DOIs
Publication statusPublished - 2005 May 1

Keywords

  • Automated synthesis
  • F-18
  • FRP-170
  • Fluoromisonidazole
  • Hypoxic cell marker
  • On-column
  • Solid phase extraction

ASJC Scopus subject areas

  • Radiation

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