Autocrinological role of basic fibroblast growth factor on tube formation of vascular endothelial cells in vitro

Yasufumi Sato, Tatsuo Shimada, Ryosaburo Takaki

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)

Abstract

When bovine capillary endothelial (BCE) cells plated on type I collagen gel were covered with a second layer of collage gel, BCE cells reorganized into a network of capillary-like structures. In the presence of affinity purified anti-basic fibroblast growth factor (bFGF) antibody, this reorganization was inhibited. By using a computerized image analyzer, the formation of network structures and the effect of anti-bFGF antibody was quantitated. The inhibtory effect of anti-FGF antibody was dose -dependent and maximal inhibition was observed at 2.0 μg/ml of antibody. Exogenously added bFGF potentiated network formation of BCE cells and coadministration of bFGF abrogated the inhibitory effect of anti-bFGF antibody. Platelet factor 4, which blocks the binding of bFGF to its receptor, inhibited network formation. These results indicate that bFGF produced by endothelial cells regulates angiogenesis as an autocrine factor.

Original languageEnglish
Pages (from-to)1098-1102
Number of pages5
JournalBiochemical and biophysical research communications
Volume180
Issue number2
DOIs
Publication statusPublished - 1991 Oct 31
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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