TY - JOUR
T1 - Autocrinological role of basic fibroblast growth factor on tube formation of vascular endothelial cells in vitro
AU - Sato, Yasufumi
AU - Shimada, Tatsuo
AU - Takaki, Ryosaburo
PY - 1991/10/31
Y1 - 1991/10/31
N2 - When bovine capillary endothelial (BCE) cells plated on type I collagen gel were covered with a second layer of collage gel, BCE cells reorganized into a network of capillary-like structures. In the presence of affinity purified anti-basic fibroblast growth factor (bFGF) antibody, this reorganization was inhibited. By using a computerized image analyzer, the formation of network structures and the effect of anti-bFGF antibody was quantitated. The inhibtory effect of anti-FGF antibody was dose -dependent and maximal inhibition was observed at 2.0 μg/ml of antibody. Exogenously added bFGF potentiated network formation of BCE cells and coadministration of bFGF abrogated the inhibitory effect of anti-bFGF antibody. Platelet factor 4, which blocks the binding of bFGF to its receptor, inhibited network formation. These results indicate that bFGF produced by endothelial cells regulates angiogenesis as an autocrine factor.
AB - When bovine capillary endothelial (BCE) cells plated on type I collagen gel were covered with a second layer of collage gel, BCE cells reorganized into a network of capillary-like structures. In the presence of affinity purified anti-basic fibroblast growth factor (bFGF) antibody, this reorganization was inhibited. By using a computerized image analyzer, the formation of network structures and the effect of anti-bFGF antibody was quantitated. The inhibtory effect of anti-FGF antibody was dose -dependent and maximal inhibition was observed at 2.0 μg/ml of antibody. Exogenously added bFGF potentiated network formation of BCE cells and coadministration of bFGF abrogated the inhibitory effect of anti-bFGF antibody. Platelet factor 4, which blocks the binding of bFGF to its receptor, inhibited network formation. These results indicate that bFGF produced by endothelial cells regulates angiogenesis as an autocrine factor.
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U2 - 10.1016/S0006-291X(05)81179-9
DO - 10.1016/S0006-291X(05)81179-9
M3 - Article
C2 - 1719962
AN - SCOPUS:0025885692
VL - 180
SP - 1098
EP - 1102
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -