Autocrine stimulation of interleukin 1β in acute myelogenous leukemia cells

K. Sakai; T. Hattori; M. Matsuoka; N. Asou; S. Yamamoto; K. Sagawa; K. Takatsuki

Autocrine stimulation of interleukin 1β in acute myelogenous leukemia cells

K. Sakai, T. Hattori, M. Matsuoka, N. Asou, S. Yamamoto, K. Sagawa, K. Takatsuki

Research output: Contribution to journal › Article › peer-review

Abstract

A significant increase in CD25 antigen-positive cells by IL-1 was observed in cells of a patient with M7 acute myelogenous leukemia. Basal proliferation and expression of CD25 antigen by the M7 leukemic cells were inhibited by addition of anti-IL-1β antibody in a dose-dependent manner, but not by rabbit anti-IL-1α antibody. Culture supernatants of these leukemic cells contained IL-1 activity, which was specifically inhibited by addition of anti-IL-1β antibody, and Northern blot analysis detected intracellular IL-1β mRNA. These results indicated that autocrine secretion of IL-1β was involved in proliferation of some myelogenous leukemic cells.

Original language	English
Pages (from-to)	1597-1602
Number of pages	6
Journal	Journal of Experimental Medicine
Volume	166
Issue number	5
Publication status	Published - 1987

ASJC Scopus subject areas

Immunology

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title = "Autocrine stimulation of interleukin 1β in acute myelogenous leukemia cells",

abstract = "A significant increase in CD25 antigen-positive cells by IL-1 was observed in cells of a patient with M7 acute myelogenous leukemia. Basal proliferation and expression of CD25 antigen by the M7 leukemic cells were inhibited by addition of anti-IL-1β antibody in a dose-dependent manner, but not by rabbit anti-IL-1α antibody. Culture supernatants of these leukemic cells contained IL-1 activity, which was specifically inhibited by addition of anti-IL-1β antibody, and Northern blot analysis detected intracellular IL-1β mRNA. These results indicated that autocrine secretion of IL-1β was involved in proliferation of some myelogenous leukemic cells.",

author = "K. Sakai and T. Hattori and M. Matsuoka and N. Asou and S. Yamamoto and K. Sagawa and K. Takatsuki",

year = "1987",

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pages = "1597--1602",

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T1 - Autocrine stimulation of interleukin 1β in acute myelogenous leukemia cells

AU - Sakai, K.

AU - Hattori, T.

AU - Matsuoka, M.

AU - Asou, N.

AU - Yamamoto, S.

AU - Sagawa, K.

AU - Takatsuki, K.

PY - 1987

Y1 - 1987

N2 - A significant increase in CD25 antigen-positive cells by IL-1 was observed in cells of a patient with M7 acute myelogenous leukemia. Basal proliferation and expression of CD25 antigen by the M7 leukemic cells were inhibited by addition of anti-IL-1β antibody in a dose-dependent manner, but not by rabbit anti-IL-1α antibody. Culture supernatants of these leukemic cells contained IL-1 activity, which was specifically inhibited by addition of anti-IL-1β antibody, and Northern blot analysis detected intracellular IL-1β mRNA. These results indicated that autocrine secretion of IL-1β was involved in proliferation of some myelogenous leukemic cells.

AB - A significant increase in CD25 antigen-positive cells by IL-1 was observed in cells of a patient with M7 acute myelogenous leukemia. Basal proliferation and expression of CD25 antigen by the M7 leukemic cells were inhibited by addition of anti-IL-1β antibody in a dose-dependent manner, but not by rabbit anti-IL-1α antibody. Culture supernatants of these leukemic cells contained IL-1 activity, which was specifically inhibited by addition of anti-IL-1β antibody, and Northern blot analysis detected intracellular IL-1β mRNA. These results indicated that autocrine secretion of IL-1β was involved in proliferation of some myelogenous leukemic cells.

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