Augmentation of T H-1 type response by immunoactive AT oligonucleotide from lactic acid bacteria via Toll-like receptor 9 signaling

Takeshi Shimosato, Haruki Kitazawa, Shinichiro Katoh, Masanori Tohno, Iliyan Dimitrov Iliev, Chiho Nagasawa, Toshiro Kimura, Yasushi Kawai, Tadao Saito

    Research output: Contribution to journalArticlepeer-review

    60 Citations (Scopus)

    Abstract

    Toll-like receptor 9, which is expressed on the surface of antigen presenting cells and which was recently identified in the cytoplasmic follicle, recognizes bacterial CpG oligodeoxynucleotides (ODNs), resulting in the induction of a potent immune response. However, in our previous study, we found that TLR9 potentially recognizes not only CpG ODN but also non-CpG ODN such as AT ODN. Therefore, in the present study, to investigate this possibility, we elucidated the effects of AT ODN on T H-1, T H-2 type cytokine induction via TLR9 by real-time quantitative PCR analysis and ELISA of the swine TLR9 transfectant. The results demonstrated that the T H-1 type cytokines such as interleukin (IL)-12p70 and interferon (IFN)-γ were strongly induced by AT ODN compared to the unexposed controls, while T H-2 type cytokines were not induced. These results indicate that the AT ODN can augment the T H-1 immune response, which plays an important role in prevention of allergic responses. Moreover, the swine TLR9 transfectant demonstrated its usefulness for evaluation of immunostimulation by bacterial DNA through the detection of T H-1, T H-2 type cytokine induction via TLR9 signaling.

    Original languageEnglish
    Pages (from-to)782-787
    Number of pages6
    JournalBiochemical and biophysical research communications
    Volume326
    Issue number4
    DOIs
    Publication statusPublished - 2005 Jan 28

    Keywords

    • AT ODN
    • Swine
    • Toll-like receptor 9
    • Transfectant

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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