Augmentation of antigen-stimulated allergic responses by a small amount of trichloroethylene ingestion from drinking water

Makoto Seo, Takeo Yamagiwa, Ryo Kobayashi, Koji Ikeda, Masahiko Satoh, Naoki Inagaki, Hiroichi Nagai, Hisamitsu Nagase

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

In previous report, we have shown that trichloroethylene (TCE) increases histamine release and inflammatory cytokine production from antigen-stimulated mast cells. In this study, we examined the enhancing effect of a small amount of TCE ingestion from drinking water on antigen-stimulated allergic responses. After exposure of Wistar rats to TCE ingestion for 2 or 4 weeks, we performed a passive cutaneous anaphylaxis (PCA) reaction. TCE ingestion for 2 and 4 weeks enhanced PCA reaction in a dose-dependent manner. On histological examination, TCE ingestion for 2 weeks exacerbated inflammation characterized by infiltration of lymphocytes and accumulation of mast cells around the vessel in the skin. After TCE ingestion for 4 weeks, the mesenteric lymph nodes (MLNs) showed increase of the size and wet weight, and germinal centers changed distinctly. The interleukin-4 (IL-4) mRNA levels on spleen, MLNs and leukocytes were increased. Moreover, serum total IgE levels of TCE ingestion increased in a time-dependent manner. Our results suggest that TCE ingestion induces pro-inflammatory responses and causes Th1/Th2-type helper T-cell imbalance. And more, a small amount of TCE ingestion may lead to the initiation and acceleration of type I allergic reaction.

Original languageEnglish
Pages (from-to)140-146
Number of pages7
JournalRegulatory Toxicology and Pharmacology
Volume52
Issue number2
DOIs
Publication statusPublished - 2008 Nov 1
Externally publishedYes

Keywords

  • IL-4
  • Mast cells
  • Th1/Th2-type helper T cells
  • Total IgE
  • Trichloroethylene
  • Type I allergic reaction

ASJC Scopus subject areas

  • Toxicology

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