Attenuation of interleukin 2 signal in the spleen cells of complex ganglioside-lacking mice

Jinmin Zhao, Keiko Furukawa, Satoshi Fukumoto, Masahiko Okada, Heiko Furugen, Hiroshi Miyazaki, Kogo Takamiya, Shinichi Aizawa, Hiroshi Shiku, Toshifumi Matsuyama, Koichi Furukawa

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41 Citations (Scopus)

Abstract

T cell development and function in complex ganglioside-lacking (GM2/GD2 synthase gene-disrupted) mice were analyzed. GM1, asialo-GM1, and GD1b were representative gangliosides expressed on T cells of the wild type mice and completely deleted on those of the mutant mice. The sizes and cell numbers of the mutant mice spleen and thymus were significantly reduced. Spleen cells from the mutant mice showed clearly reduced proliferation compared with the wild type when stimulated by interleukin 2 (IL-2) but not when treated with concanavalin A or anti-CD3 cross-linking. Expression levels of IL-2 receptor α, β, and γ, were almost equivalent, and up-regulation of α chain after T cell activation was also similar between the mutant and wild type mice. Activation of JAK1, JAK3, and SAT5 after IL-2 treatment was reduced, and c- fos expression was delayed and reduced in the mutant spleen cells, suggesting that the IL-2 signal was attenuated in the mutant mice probably due to the modulation of IL-2 receptors by the lack of complex gangliosides.

Original languageEnglish
Pages (from-to)13744-13747
Number of pages4
JournalJournal of Biological Chemistry
Volume274
Issue number20
DOIs
Publication statusPublished - 1999 May 14

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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