Attenuation of Ca2+-induced increase in oxidative metabolism by cooling and calmodulin antagonist in mammalian brain neurons: a flow-cytometric study

Yasuo Oyama, Lumi Chikahisa, Hideki Matoba, Katsutoshi Furukawa

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3 Citations (Scopus)

Abstract

Effects of cooling and calmodulin antagonist on ionomycin-induced increase in oxidative metabolism (or formation of reactive oxygen species) of rat cerebellar neurons was examined using a flow cytometer and 2′,7′-dichlorofluorescin diacetate, a fluorescent dye for intracellular hydrogen peroxide. Cooling neurons to temperatures below 16°C greatly attenuated ionomycin-induced augmentation of oxidative metabolism without affecting the Ca2+ influx produced by ionomycin. Rewarming neurons to 36°C in peesence of ionomycin increased the oxidative metabolism, indicating a temperature-sensitive metabolic process. Substitution of Ca2+ with Ba2+ or Sr2+ completely abolished an ionomycin-induced increase in the oxidative metabolism. Pretreatment with W-7, a calmodulin antagonist, at concentrations of 10 μM or higher (up to 100 μM) produced a dose-dependent attenuation of ionomycin-induced increase in oxidative metabolism. Results suggest that calmodulin is involved in the ionomycin-induced increase in oxidative metabolism of dissociated cerebellar neurons.

Original languageEnglish
Pages (from-to)220-224
Number of pages5
JournalBrain research
Volume664
Issue number1-2
DOIs
Publication statusPublished - 1994 Nov 21

Keywords

  • Calmodulin
  • Central nervous system neuron
  • Flow cytometer
  • Oxidative metabolism
  • Reactive oxygen species
  • W-7

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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