Attachment duration required for Rhipicephalus appendiculatus to transmit Theileria parva to the host

Satoru Konnai, Shinji Yamada, Saiki Imamura, Martin Simuunza, Mwelwa Chembensof, Amos Chota, Andrew Nambota, Kazuhiko Ohashi, Misao Onuma

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Theileria parva, the agent of East Coast fever (ECF), is transmitted to the host during the blood meal feeding of Rhipicephalus appendiculatus ticks. In order to investigate the relationship between the attachment duration of R. appendiculatus and the transmission of T. parva, infected adult ticks were allowed to attach to naive mice for variable lengths of time. Attached ticks and host animal's back skin biopsies from the tick attachment site were collected daily, starting from 24 hours post-tick attachment, and used for seminested polymerase chain reaction (PCR) detection of T. parva. T. parva-infected ticks started to transmit the parasites from 72 hours post-tick attachment. As expected, the transmission of T. parva from ticks to mouse skin increased with duration of tick attachment. Transmission of the parasites was 77.7%, 100%, 85.5%, and 100% on day 4, 5, 6, and 7 post-tick attachment, respectively, as could be detected from mice skin biopsies taken from T. parva-infected ticks' attachment sites. These results have important implications for our understanding of early events in the transmission of T. parva and would help in the development of effective pharmacologic substances and/or vaccines against ticks.

Original languageEnglish
Pages (from-to)241-248
Number of pages8
JournalVector-Borne and Zoonotic Diseases
Volume7
Issue number2
DOIs
Publication statusPublished - 2007 Jun 1
Externally publishedYes

Keywords

  • Duration
  • Rhipicephalus appendiculatus
  • Theileria parva
  • Tick attachment
  • Transmission

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases
  • Virology

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    Konnai, S., Yamada, S., Imamura, S., Simuunza, M., Chembensof, M., Chota, A., Nambota, A., Ohashi, K., & Onuma, M. (2007). Attachment duration required for Rhipicephalus appendiculatus to transmit Theileria parva to the host. Vector-Borne and Zoonotic Diseases, 7(2), 241-248. https://doi.org/10.1089/vbz.2006.0616